X-Ray Diffraction data from Escherichia coli DOS Br complex, source of 1V9Z structure

Data DOI: 10.15785/SBGRID/137 | ID: 137

Publication DOI: 10.1074/jbc.M314199200

1V9Z Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Raman Laboratory, University of Maryland

Release Date: June 5, 2015

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/137

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/137 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/137 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/137 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/137 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 137 ; shasum -c files.sha'

Storage requirements: 126M

Biological Sample:

Escherichia coli DOS Br complex

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

BL 9-2, Stanford Synchrotron Radiation Laboratory

Data Creation Date:

Jan. 5, 2003

Related Datasets:

133, 134, 135, 136


Cite this Dataset

Lee, D; Raman, Cs. 2015. "X-Ray Diffraction data for: Escherichia coli DOS Br complex. PDB Code 1V9Z", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/137.

Download Citation

Dataset Description

DOS crystals quick-soaked (10 s) with 0.5 KBr were used for data collection at 1.08 A . f" (0.6) is not optimal at this wavelength. Nonetheless, the data yield useful information regarding binding sites.

Project Members

Name Additional Roles Affiliation While Working on the Project
C s RamanData Collector, DepositorUniversity of Texas Medical School
Dong-Sun LeeData CollectorUniversity of Texas Medical School
C S RamanPIUniversity of Maryland

Reprocessing Instructions

XDS/HKL2000/MOSFLM all work well.

Reprocessing Data

About Reprocessing

Version:

version unreported

Reprocessing failed.

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 1.08

Sweep:

SWEEP1

Files:

/programs/datagrid/137/dosBr_###.mar1200

Images:

1 - 103

Beam:

(90.0, 90.0) -> (90.0, 91.07)

Distance:

140.0 -> 139.21

Spacegroup:

P 21 21 21

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.91 5.18 1.91
Low resolution limit 28.28 28.28 1.94
Completeness 98.5 98.4 75.0
Multiplicity 4.0 3.6 2.5
I/sigma 21.9 50.9 2.7
Rmerge 0.031 0.014 0.257
CC half 0.999 0.999 0.854
Anomalous completeness 94.9 93.6 49.2
Anomalous multiplicity 2.2 2.2 2.2
Cell 47.08 68.69 82.52 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 1.08

Sweep:

SWEEP1

Files:

/programs/datagrid/137/dosBr_###.mar1200

Images:

1 - 103

Beam:

(90.0, 90.0) -> (90.0, 91.08)

Distance:

140.0 -> 139.26

Spacegroup:

P 21 21 21

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.91 5.18 1.91
Low resolution limit 28.28 28.28 1.94
Completeness 98.5 98.4 75.0
Multiplicity 4.0 3.6 2.5
I/sigma 21.9 50.9 2.7
Rmerge 0.031 0.014 0.255
CC half 0.999 0.999 0.859
Anomalous completeness 94.9 93.6 49.2
Anomalous multiplicity 2.2 2.2 2.2
Cell 47.08 68.69 82.52 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 1.08

Sweep:

SWEEP1

Files:

/programs/datagrid/137/dosBr_###.mar1200

Images:

1 - 103

Beam:

(90.0, 90.0) -> (90.0, 91.07)

Distance:

140.0 -> 139.11

Spacegroup:

P 21 21 21

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.91 5.18 1.91
Low resolution limit 27.69 27.7 1.94
Completeness 98.4 98.5 67.7
Multiplicity 3.9 3.7 2.4
I/sigma 14.1 31.4 2.3
Rmerge 0.05 0.026 0.193
CC half 0.995 0.998 0.553
Anomalous completeness 96.5 98.1 42.2
Anomalous multiplicity 2.1 2.1 2.1
Cell 46.994 68.555 82.358 90.0 90.0 90.0

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.