X-Ray Diffraction data from Escherichia coli DOS reduced with dithionite and treated with nitric oxide, source of 1V9Z structure

Data DOI: 10.15785/SBGRID/135 | ID: 135

Publication DOI: 10.1074/jbc.M314199200

1V9Z Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Raman Laboratory, University of Maryland

Release Date: June 5, 2015

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/135

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/135 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/135 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/135 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/135 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 135 ; shasum -c files.sha'

Storage requirements: 2.0G

Biological Sample:

Escherichia coli DOS reduced with dithionite and treated with nitric oxide

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

BL 9-2, Stanford Synchrotron Radiation Laboratory

Data Creation Date:

July 10, 2002

Related Datasets:

133, 134, 136, 137


Cite this Dataset

Lee, D; Raman, CS. 2015. "X-Ray Diffraction data for: Escherichia coli DOS reduced with dithionite and treated with nitric oxide. PDB Code 1V9Z", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/135.

Download Citation

Dataset Description

This is data collected from a DOS crystal that was first reduced with sodium dithionite and then soaked in a solution of nitric oxide.

Project Members

Name Additional Roles Affiliation While Working on the Project
C S RamanData Collector, DepositorUniversity of Texas Medical School
Dong-Sun LeeData CollectorUniversity of Texas Medical School
C S RamanPIUniversity of Maryland

Reprocessing Instructions

should process fine with XDS/HKL2000/MOSFLM

Reprocessing Data

About Reprocessing

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.98397

Sweep:

SWEEP1

Files:

/programs/datagrid/135/dosNO_4_###.img

Images:

1 - 110

Beam:

(157.5, 157.5) -> (157.5, 157.47)

Distance:

92.0 -> 92.65

Spacegroup:

P 21 21 21

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.76 4.77 1.76
Low resolution limit 28.08 28.08 1.79
Completeness 99.7 96.8 99.5
Multiplicity 4.2 4.0 4.0
I/sigma 8.1 26.0 0.8
Rmerge 0.126 0.036 1.66
CC half 0.995 0.998 0.314
Anomalous completeness 98.1 96.6 97.1
Anomalous multiplicity 2.3 2.3 2.3
Cell 46.155 68.72 82.543 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.98397

Sweep:

SWEEP1

Files:

/programs/datagrid/135/dosNO_4_###.img

Images:

1 - 110

Beam:

(157.5, 157.5) -> (157.5, 157.44)

Distance:

92.0 -> 92.47

Spacegroup:

P 21 21 21

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.29 6.2 2.29
Low resolution limit 34.33 34.34 2.33
Completeness 99.7 94.3 100.0
Multiplicity 4.4 3.8 4.5
I/sigma 17.0 32.1 6.1
Rmerge 0.055 0.028 0.255
CC half 0.996 0.997 0.962
Anomalous completeness 98.3 86.4 99.0
Anomalous multiplicity 2.4 2.4 2.4
Cell 46.11 68.67 82.34 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.98397

Sweep:

SWEEP1

Files:

/programs/datagrid/135/dosNO_4_###.img

Images:

1 - 110

Beam:

(157.5, 157.5) -> (157.5, 157.43)

Distance:

92.0 -> 92.53

Spacegroup:

P 21 21 21

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.86 5.04 1.86
Low resolution limit 34.67 34.67 1.89
Completeness 99.8 96.8 99.8
Multiplicity 4.4 4.0 4.3
I/sigma 10.5 31.0 1.1
Rmerge 0.087 0.03 1.238
CC half 0.996 0.997 0.601
Anomalous completeness 98.7 91.9 98.6
Anomalous multiplicity 2.4 2.4 2.4
Cell 46.05 68.58 82.25 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.98397

Sweep:

SWEEP1

Files:

/programs/datagrid/135/dosNO_4_###.img

Images:

1 - 110

Beam:

(157.5, 157.5) -> (157.5, 157.42)

Distance:

92.0 -> 92.51

Spacegroup:

P 21 21 21

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.79 4.86 1.79
Low resolution limit 34.79 34.8 1.82
Completeness 99.6 96.3 98.6
Multiplicity 4.2 4.0 3.9
I/sigma 4.1 8.5 1.1
Rmerge 0.147 0.049 0.973
CC half 0.99 0.996 0.102
Anomalous completeness 97.6 96.6 95.6
Anomalous multiplicity 2.3 2.3 2.3
Cell 46.224 68.825 82.487 90.0 90.0 90.0

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.