Data DOI: 10.15785/SBGRID/135 | ID: 135
Publication DOI: 10.1074/jbc.M314199200
1V9Z Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB
Raman Laboratory, University of Maryland
Release Date: 5 Jun 2015
1. If this dataset is locally available, it should be accessable at /programs/datagrid/135
2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:
'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/135 .' (Harvard Medical School, USA)
Depending on your location, faster access may be available from a Tier 1 site closer to your location
'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/135 .' (Uppsala University, Sweden)
'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/135 .' (Institut Pasteur de Montevideo, Uruguay)
'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/135 .' (Shanghai Institutes for Biological Sciences, China)
3. After the transfer is completed, please issue the following command to verify data integrity:
'cd 135 ; shasum -c files.sha'
Storage requirements: 2.0G
Biological Sample:
Escherichia coli DOS reduced with dithionite and treated with nitric oxide
Dataset Type:
X-Ray Diffraction
Subject Composition:
Protein
Collection Facility:
BL 9-2, Stanford Synchrotron Radiation Laboratory
Data Creation Date:
10 Jul 2002
Related Datasets:
Lee, D; Raman, CS. 2015. "X-Ray Diffraction data for: Escherichia coli DOS reduced with dithionite and treated with nitric oxide. PDB Code 1V9Z", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/135.
This is data collected from a DOS crystal that was first reduced with sodium dithionite and then soaked in a solution of nitric oxide.
| Name | Additional Roles | Affiliation While Working on the Project |
|---|---|---|
| C S Raman | Data Collector, Depositor | University of Texas Medical School |
| Dong-Sun Lee | Data Collector | University of Texas Medical School |
| C S Raman | PI | University of Maryland |
should process fine with XDS/HKL2000/MOSFLM
Version:
version unreported
Project:
AUTOMATIC
Crystal:
DEFAULT
Sequence Length:
0
Wavelength:
SAD - 0.98397
Sweep:
SWEEP1
Files:
/programs/datagrid/135/dosNO_4_###.img
Images:
1 - 110
Beam:
(157.5, 157.5) -> (157.5, 157.44)
Distance:
92.0 -> 92.47
Spacegroup:
P 21 21 21
For AUTOMATIC/DEFAULT/SAD:
| ALL | LOW | HIGH | |
|---|---|---|---|
| High resolution limit | 1.74 | 4.72 | 1.74 |
| Low resolution limit | 34.32 | 34.33 | 1.77 |
| Completeness | 99.8 | 96.2 | 100.0 |
| Multiplicity | 4.4 | 4.0 | 4.3 |
| I/sigma | 8.7 | 31.6 | 0.6 |
| Rmerge | 0.104 | 0.029 | 2.415 |
| CC half | 0.997 | 0.998 | 0.267 |
| Anomalous completeness | 98.8 | 95.1 | 98.9 |
| Anomalous multiplicity | 2.3 | 2.3 | 2.3 |
| Cell | 46.08 | 68.64 | 82.31 | 90.0 | 90.0 | 90.0 |
|---|
Version:
version unreported
Project:
AUTOMATIC
Crystal:
DEFAULT
Sequence Length:
0
Wavelength:
SAD - 0.98397
Sweep:
SWEEP1
Files:
/programs/datagrid/135/dosNO_4_###.img
Images:
1 - 110
Beam:
(157.5, 157.5) -> (157.5, 157.43)
Distance:
92.0 -> 92.53
Spacegroup:
P 21 21 21
For AUTOMATIC/DEFAULT/SAD:
| ALL | LOW | HIGH | |
|---|---|---|---|
| High resolution limit | 1.74 | 4.72 | 1.74 |
| Low resolution limit | 34.61 | 34.61 | 1.77 |
| Completeness | 99.8 | 96.2 | 100.0 |
| Multiplicity | 4.4 | 4.0 | 4.3 |
| I/sigma | 8.8 | 31.6 | 0.6 |
| Rmerge | 0.104 | 0.029 | 2.525 |
| CC half | 0.997 | 0.998 | 0.251 |
| Anomalous completeness | 98.8 | 94.8 | 98.7 |
| Anomalous multiplicity | 2.3 | 2.3 | 2.3 |
| Cell | 45.97 | 68.44 | 82.11 | 90.0 | 90.0 | 90.0 |
|---|
Version:
version unreported
Project:
AUTOMATIC
Crystal:
DEFAULT
Sequence Length:
0
Wavelength:
SAD - 0.98397
Sweep:
SWEEP1
Files:
/programs/datagrid/135/dosNO_4_###.img
Images:
1 - 110
Beam:
(157.5, 157.5) -> (157.5, 157.42)
Distance:
92.0 -> 92.45
Spacegroup:
P 21 21 21
For AUTOMATIC/DEFAULT/SAD:
| ALL | LOW | HIGH | |
|---|---|---|---|
| High resolution limit | 1.79 | 4.85 | 1.79 |
| Low resolution limit | 23.62 | 23.63 | 1.82 |
| Completeness | 99.9 | 100.0 | 98.2 |
| Multiplicity | 4.3 | 4.0 | 4.1 |
| I/sigma | 10.3 | 39.2 | 1.7 |
| Rmerge | 0.142 | 0.081 | 0.617 |
| CC half | 0.954 | 0.918 | 0.249 |
| Anomalous completeness | 98.4 | 99.9 | 96.6 |
| Anomalous multiplicity | 2.3 | 2.3 | 2.3 |
| Cell | 46.208 | 68.844 | 82.462 | 90.0 | 90.0 | 90.0 |
|---|
License: CC0
Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.
