MicroED structure of a FIB-milled CypA Crystal; Tamir Gonen, Michael C Thompson, and James Fraser are co-PIs of this dataset.
Data DOI: 10.15785/SBGRID/752 | PDB ID 6U5G: RCSB PDBe | Published: 28 Jan 2020
Fraser Laboratory, University of California, San Francisco
Trypsin crystals were obtained according to the method of Liebschner et.al (Liebschner et al., 2013). Lyophilized bovine pancreas trypsin was purchased from Sigma-Aldrich (T1005) and dissolved at a concentration of 30 mg/mL into 30mM HEPES pH 7.0, 5 mg/mL benzamidine and 3mM CaCl2. Crystals were obtained from a solution of 200mM Ammonium sulfate, 100mM Na cacodylate pH 6.5, 20% PEG 8000 and 15% glycerol. Data collected for simultaneous Bragg and Diffuse Scattering processing.
Data DOI: 10.15785/SBGRID/201 | PDB ID 5F6M: RCSB PDBe | Published: 18 Dec 2015
Fraser Laboratory, University of California, San Francisco
Highest resolution room temperature diffraction data set for Cyclophilin A. Collected under paratone oil.
Data DOI: 10.15785/SBGRID/68 | PDB ID 4YUO: RCSB PDBe | Publication DOI: 10.1101/016733 | Published: 8 May 2015
Fraser Laboratory, University of California, San Francisco