Data DOI: 10.15785/SBGRID/201 | ID: 201
5F6M Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB
Fraser Laboratory, University of California, San Francisco
Release Date: 18 Dec 2015
1. If this dataset is locally available, it should be accessable at /programs/datagrid/201
2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:
'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/201 .' (Harvard Medical School, USA)
Depending on your location, faster access may be available from a Tier 1 site closer to your location
'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/201 .' (Uppsala University, Sweden)
'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/201 .' (Institut Pasteur de Montevideo, Uruguay)
'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/201 .' (Shanghai Institutes for Biological Sciences, China)
3. After the transfer is completed, please issue the following command to verify data integrity:
'cd 201 ; shasum -c files.sha'
Storage requirements: 703M
Biological Sample:
BOVINE TRYPSIN
Dataset Type:
X-Ray Diffraction
Subject Composition:
Protein
Collection Facility:
SSRL BL 11-1
Data Creation Date:
16 Jan 2014
Related Datasets:
None
Van Benschoten, AH; Fraser, JS. 2015. "X-Ray Diffraction data for: BOVINE TRYPSIN. PDB Code 5F6M", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/201.
Trypsin crystals were obtained according to the method of Liebschner et.al (Liebschner et al., 2013). Lyophilized bovine pancreas trypsin was purchased from Sigma-Aldrich (T1005) and dissolved at a concentration of 30 mg/mL into 30mM HEPES pH 7.0, 5 mg/mL benzamidine and 3mM CaCl2. Crystals were obtained from a solution of 200mM Ammonium sulfate, 100mM Na cacodylate pH 6.5, 20% PEG 8000 and 15% glycerol. Data collected for simultaneous Bragg and Diffuse Scattering processing.
| Name | Additional Roles | Affiliation While Working on the Project |
|---|---|---|
| James S Fraser | Depositor | UCSF |
| Andrew H Van Benschoten | Data Collector | UCSF |
| James Fraser | PI | University of California, San Francisco |
none
Version:
version unreported
Project:
AUTOMATIC
Crystal:
DEFAULT
Sequence Length:
0
Wavelength:
SAD - 1.03317
Sweep:
SWEEP1
Files:
/programs/datagrid/201/crystal3_#####.cbf
Images:
103 - 118
Beam:
(217.32, 211.82) -> (217.32, 211.71)
Distance:
200.0 -> 199.61
Spacegroup:
P 21 21 2
For AUTOMATIC/DEFAULT/SAD:
| ALL | LOW | HIGH | |
|---|---|---|---|
| High resolution limit | 1.19 | 3.23 | 1.19 |
| Low resolution limit | 19.99 | 19.99 | 1.21 |
| Completeness | 33.0 | 31.1 | 20.0 |
| Multiplicity | 1.6 | 1.8 | 1.1 |
| I/sigma | 9.2 | 31.3 | 1.3 |
| Rmerge | 0.022 | 0.016 | 0.168 |
| CC half | 0.998 | 0.997 | 0.828 |
| Anomalous completeness | 17.9 | 19.7 | 1.4 |
| Anomalous multiplicity | 1.1 | 1.1 | 1.1 |
| Cell | 54.79 | 58.48 | 67.4 | 90.0 | 90.0 | 90.0 |
|---|
Version:
version unreported
Project:
AUTOMATIC
Crystal:
DEFAULT
Sequence Length:
0
Wavelength:
SAD - 1.03317
Sweep:
SWEEP1
Files:
/programs/datagrid/201/crystal3_#####.cbf
Images:
103 - 118
Beam:
(217.32, 211.82) -> (217.32, 211.71)
Distance:
200.0 -> 199.61
Spacegroup:
P 21 21 2
For AUTOMATIC/DEFAULT/SAD:
| ALL | LOW | HIGH | |
|---|---|---|---|
| High resolution limit | 1.19 | 3.23 | 1.19 |
| Low resolution limit | 19.99 | 19.99 | 1.21 |
| Completeness | 33.0 | 31.1 | 20.0 |
| Multiplicity | 1.6 | 1.8 | 1.1 |
| I/sigma | 9.2 | 31.1 | 1.2 |
| Rmerge | 0.022 | 0.016 | 0.188 |
| CC half | 0.998 | 0.997 | 0.83 |
| Anomalous completeness | 17.9 | 19.6 | 1.4 |
| Anomalous multiplicity | 1.1 | 1.1 | 1.1 |
| Cell | 54.79 | 58.48 | 67.4 | 90.0 | 90.0 | 90.0 |
|---|
Version:
version unreported
Project:
AUTOMATIC
Crystal:
DEFAULT
Sequence Length:
0
Wavelength:
SAD - 1.03317
Sweep:
SWEEP1
Files:
/programs/datagrid/201/crystal3_#####.cbf
Images:
103 - 118
Beam:
(217.32, 211.82) -> (217.32, 211.7)
Distance:
200.0 -> 199.77
Spacegroup:
P 21 21 2
For AUTOMATIC/DEFAULT/SAD:
| ALL | LOW | HIGH | |
|---|---|---|---|
| High resolution limit | 1.18 | 3.2 | 1.18 |
| Low resolution limit | 24.82 | 24.82 | 1.2 |
| Completeness | 32.8 | 31.8 | 18.5 |
| Multiplicity | 1.6 | 1.8 | 1.0 |
| I/sigma | 17.2 | 73.1 | 1.7 |
| Rmerge | 0.024 | 0.02 | 0.082 |
| CC half | 0.997 | 0.995 | 0.816 |
| Anomalous completeness | 17.5 | 20.3 | 0.7 |
| Anomalous multiplicity | 1.1 | 1.1 | 1.1 |
| Cell | 54.808 | 58.501 | 67.431 | 90.0 | 90.0 | 90.0 |
|---|
License: CC0
Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.
