X-Ray Diffraction data from BOVINE TRYPSIN, source of 5F6M structure

Data DOI: 10.15785/SBGRID/201 | ID: 201

5F6M Coordinates: Viewer, PDB, MMDB

Fraser Laboratory, University of California, San Francisco

Release Date: 18 Dec 2015

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/201

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/201 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/201 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/201 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/201 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 201 ; shasum -c files.sha'

Storage requirements: 703M

Biological Sample:

BOVINE TRYPSIN

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

SSRL BL 11-1

Data Creation Date:

16 Jan 2014

Related Datasets:

None


Cite this Dataset

Van Benschoten, AH; Fraser, JS. 2015. "X-Ray Diffraction data for: BOVINE TRYPSIN. PDB Code 5F6M", SBGrid Data Bank, V1, http://dx.doi.org/10.15785/SBGRID/201.

Download Citation

Dataset Description

Trypsin crystals were obtained according to the method of Liebschner et.al (Liebschner et al., 2013). Lyophilized bovine pancreas trypsin was purchased from Sigma-Aldrich (T1005) and dissolved at a concentration of 30 mg/mL into 30mM HEPES pH 7.0, 5 mg/mL benzamidine and 3mM CaCl2. Crystals were obtained from a solution of 200mM Ammonium sulfate, 100mM Na cacodylate pH 6.5, 20% PEG 8000 and 15% glycerol. Data collected for simultaneous Bragg and Diffuse Scattering processing.

Project Members

Name Additional Roles Affiliation While Working on the Project
James S FraserDepositorUCSF
Andrew H Van BenschotenData CollectorUCSF
James FraserPIUniversity of California, San Francisco

Reprocessing Instructions

none

Reprocessing Data

About Reprocessing

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 1.03317

Sweep:

SWEEP1

Files:

/programs/datagrid/201/crystal3_#####.cbf

Images:

103 - 118

Beam:

(217.32, 211.82) -> (217.32, 211.86)

Distance:

200.0 -> 199.82

Spacegroup:

P 21 21 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.18 3.2 1.18
Low resolution limit 24.81 24.81 1.2
Completeness 30.8 30.9 15.4
Multiplicity 1.5 1.7 1.0
I/sigma 10.0 34.2 1.5
Rmerge 0.023 0.018 0.299
CC half 0.998 0.998 0.678
Anomalous completeness 14.5 18.4 0.3
Anomalous multiplicity 1.1 1.1 1.1
Cell 54.793 58.486 67.424 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 1.03317

Sweep:

SWEEP1

Files:

/programs/datagrid/201/crystal3_#####.cbf

Images:

103 - 118

Beam:

(217.32, 211.82) -> (217.32, 211.71)

Distance:

200.0 -> 200.17

Spacegroup:

P 21 21 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.22 3.31 1.22
Low resolution limit 20.03 20.03 1.24
Completeness 33.9 31.3 24.3
Multiplicity 1.6 1.8 1.2
I/sigma 10.1 39.1 1.1
Rmerge 0.022 0.016 0.571
CC half 0.998 0.997 0.73
Anomalous completeness 19.1 19.7 4.1
Anomalous multiplicity 1.1 1.1 1.1
Cell 54.88 58.59 67.61 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 1.03317

Sweep:

SWEEP1

Files:

/programs/datagrid/201/crystal3_#####.cbf

Images:

103 - 118

Beam:

(217.32, 211.82) -> (217.32, 211.71)

Distance:

200.0 -> 200.17

Spacegroup:

P 21 21 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.22 3.31 1.22
Low resolution limit 20.03 20.03 1.24
Completeness 33.9 31.4 24.3
Multiplicity 1.6 1.8 1.2
I/sigma 10.2 38.3 1.2
Rmerge 0.022 0.017 0.57
CC half 0.998 0.997 0.73
Anomalous completeness 19.1 19.7 4.1
Anomalous multiplicity 1.1 1.1 1.1
Cell 54.88 58.59 67.62 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 1.03317

Sweep:

SWEEP1

Files:

/programs/datagrid/201/crystal3_#####.cbf

Images:

103 - 118

Beam:

(217.32, 211.82) -> (217.32, 211.7)

Distance:

200.0 -> 199.77

Spacegroup:

P 21 21 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.18 3.2 1.18
Low resolution limit 24.82 24.82 1.2
Completeness 31.9 31.3 17.1
Multiplicity 1.6 1.8 1.0
I/sigma 9.8 34.0 1.7
Rmerge 0.023 0.018 0.068
CC half 0.999 0.998 0.796
Anomalous completeness 16.4 19.6 0.4
Anomalous multiplicity 1.1 1.1 1.1
Cell 54.808 58.502 67.436 90.0 90.0 90.0

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.