X-Ray Diffraction data from Lin28/let-7d microRNA complex, source of 3TRZ structure

Data DOI: 10.15785/SBGRID/4 | ID: 4

Publication DOI: 10.1016/ j.cell.2011.10.020

3TRZ Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Sliz Laboratory, Harvard Medical School

Release Date: April 13, 2015

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/4

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/4 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/4 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/4 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/4 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 4 ; shasum -c files.sha'

Storage requirements: 1.3G

Biological Sample:

Lin28/let-7d microRNA complex

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein, RNA

Collection Facility:

NSLS X25

Data Creation Date:

Sept. 8, 2010

Related Datasets:

3


Cite this Dataset

Nam, Y; Sliz, P. 2015. "X-Ray Diffraction data for: Lin28/let-7d microRNA complex. PDB Code 3TRZ", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/4.

Download Citation

Dataset Description

Native dataset

Project Members

Name Additional Roles Affiliation While Working on the Project
Yunsun NamData CollectorHarvard Medical School
Piotr SlizDepositor, PIHarvard Medical School

Reprocessing Instructions

none

Reprocessing Data

About Reprocessing

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 1.0

Sweep:

SWEEP1

Files:

/programs/datagrid/4/yn152_4_###.img

Images:

1 - 70

Beam:

(158.0, 158.0) -> (158.0, 157.61)

Distance:

400.0 -> 401.01

Spacegroup:

C 2 2 21

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.72 7.38 2.72
Low resolution limit 112.24 112.34 2.77
Completeness 95.4 87.1 96.1
Multiplicity 2.9 3.0 2.8
I/sigma 10.4 23.6 1.1
Rmerge 0.076 0.021 1.073
CC half 0.995 0.999 0.324
Anomalous completeness 83.2 82.2 82.9
Anomalous multiplicity 1.6 1.6 1.6
Cell 204.078 204.588 178.242 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 1.0

Sweep:

SWEEP1

Files:

/programs/datagrid/4/yn152_4_###.img

Images:

1 - 70

Beam:

(158.0, 158.0) -> (158.0, 157.58)

Distance:

400.0 -> 398.79

Spacegroup:

P 4 21 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.67 7.25 2.67
Low resolution limit 75.63 75.66 2.72
Completeness 98.7 92.6 99.7
Multiplicity 5.7 5.4 5.6
I/sigma 16.1 40.2 1.2
Rmerge 0.076 0.022 1.585
CC half 0.998 0.999 0.409
Anomalous completeness 98.3 94.3 96.4
Anomalous multiplicity 3.0 3.0 3.0
Cell 143.88 143.88 177.8 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 1.0

Sweep:

SWEEP1

Files:

/programs/datagrid/4/yn152_4_###.img

Images:

1 - 70

Beam:

(158.0, 158.0) -> (158.0, 157.58)

Distance:

400.0 -> 399.45

Spacegroup:

P 4 21 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.68 7.27 2.68
Low resolution limit 75.63 75.66 2.73
Completeness 98.7 92.8 99.7
Multiplicity 5.7 5.4 5.7
I/sigma 16.3 40.3 1.2
Rmerge 0.075 0.022 1.541
CC half 0.998 0.999 0.428
Anomalous completeness 98.4 94.5 97.9
Anomalous multiplicity 3.0 3.0 3.0
Cell 143.88 143.88 177.8 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 1.0

Sweep:

SWEEP1

Files:

/programs/datagrid/4/yn152_4_###.img

Images:

1 - 70

Beam:

(158.0, 158.0) -> (158.0, 157.58)

Distance:

400.0 -> 395.18

Spacegroup:

P 4 21 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.68 7.28 2.68
Low resolution limit 87.89 87.94 2.73
Completeness 98.5 94.0 88.9
Multiplicity 5.7 5.4 5.7
I/sigma 9.2 17.6 1.1
Rmerge 0.103 0.029 1.725
CC half 0.995 0.999 0.325
Anomalous completeness 98.8 96.0 88.8
Anomalous multiplicity 3.0 3.0 3.0
Cell 143.264 143.264 176.787 90.0 90.0 90.0

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.