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X-Ray Diffraction data from Lin28/let-7d microRNA complex, source of 3TRZ structure

Data DOI: 10.15785/SBGRID/3 | ID: 3

Publication DOI: 10.1016/j.cell.2011.10.020

3TRZ Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Sliz Laboratory, Harvard Medical School

Release Date: April 13, 2015

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/3

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/3 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/3 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/3 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/3 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 3 ; shasum -c files.sha'

Storage requirements: 2.0G

Biological Sample:

Lin28/let-7d microRNA complex

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein, RNA

Collection Facility:

NSLS X25

Data Creation Date:

Sept. 7, 2010

Related Datasets:

4


Cite this Dataset

Nam, Y; Sliz, P. 2015. "X-Ray Diffraction data for: Lin28/let-7d microRNA complex. PDB Code 3TRZ", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/3.

Download Citation

Dataset Description

Zn-SAD dataset

Project Members

Name Additional Roles Affiliation While Working on the Project
Yunsun NamData CollectorHarvard Medical School
Piotr SlizDepositor, PIHarvard Medical School

Reprocessing Instructions

none

Reprocessing Data

About Reprocessing

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 1.2549

Sweep:

SWEEP1

Files:

/programs/datagrid/3/yn112_2_###.img

Images:

1 - 110

Beam:

(158.0, 158.0) -> (158.0, 157.63)

Distance:

350.0 -> 351.26

Spacegroup:

P 41 21 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.9 7.87 2.9
Low resolution limit 89.47 89.52 2.95
Completeness 100.0 100.0 99.0
Multiplicity 8.6 7.9 6.4
I/sigma 16.0 43.9 0.8
Rmerge 0.123 0.025 2.166
CC half 0.999 1.0 0.367
Anomalous completeness 99.9 99.6 98.8
Anomalous multiplicity 4.6 4.6 4.6
Cell 144.05 144.05 178.95 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 1.2549

Sweep:

SWEEP1

Files:

/programs/datagrid/3/yn112_2_###.img

Images:

1 - 110

Beam:

(158.0, 158.0) -> (158.0, 157.63)

Distance:

350.0 -> 351.5

Spacegroup:

P 41 21 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.9 7.87 2.9
Low resolution limit 101.86 101.92 2.95
Completeness 100.0 100.0 99.0
Multiplicity 8.6 7.9 6.4
I/sigma 16.0 44.0 0.8
Rmerge 0.123 0.025 2.162
CC half 0.999 1.0 0.368
Anomalous completeness 99.9 99.6 98.8
Anomalous multiplicity 4.6 4.6 4.6
Cell 144.05 144.05 178.95 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 1.2549

Sweep:

SWEEP1

Files:

/programs/datagrid/3/yn112_2_###.img

Images:

1 - 110

Beam:

(158.0, 158.0) -> (158.0, 157.63)

Distance:

350.0 -> 348.99

Spacegroup:

P 41 21 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.94 7.98 2.94
Low resolution limit 88.97 89.01 2.99
Completeness 100.0 100.0 99.9
Multiplicity 8.8 8.0 7.6
I/sigma 9.4 32.9 0.5
Rmerge 0.132 0.042 1.902
CC half 0.998 0.998 0.707
Anomalous completeness 100.0 99.9 99.8
Anomalous multiplicity 4.7 4.7 4.7
Cell 143.241 143.241 177.93 90.0 90.0 90.0
This dataset is part of the SBDB Pilot Reference Collection, and has processed intensities available from that collection.

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.