X-Ray Diffraction data from Human Protocadherin-15 EC3-5, source of 5T4M structure

Data DOI: 10.15785/SBGRID/361 | ID: 361

5T4M Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Gaudet Laboratory, Harvard University

Release Date: Feb. 14, 2017

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/361

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/361 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/361 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/361 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/361 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 361 ; shasum -c files.sha'

Storage requirements: 2.7G

Biological Sample:

Human Protocadherin-15 EC3-5

Dataset Type:

X-Ray Diffraction

Subject Composition:

Ligand, Protein

Collection Facility:

24-ID-C, NE-CAT, APS, Chicago, IL

Data Creation Date:

Aug. 8, 2013

Related Datasets:

362


Cite this Dataset

Powers, RE; Sotomayor, M; Gaudet, R. 2017. "X-Ray Diffraction data for: Human Protocadherin-15 EC3-5. PDB Code 5T4M", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/361.

Download Citation

Dataset Description

Native dataset of Human Protocadherin-15 EC3-5 used to determine structure using molecular replacement.

Project Members

Name Additional Roles Affiliation While Working on the Project
Robert E PowersData Collector, DepositorBiophysics Graduate Program, Harvard University, Boston MA 02115
Rachelle GaudetDepartment of Molecular and Cellular Biology, Harvard University, Cambridge MA 02138
Marcos SotomayorDepartment of Chemistry and Biochemistry, The Ohio State University, Columbus OH 43210
Rachelle GaudetPIHarvard University

Reprocessing Instructions

none

Reprocessing Data

About Reprocessing

Version:

version unreported

Reprocessing failed.

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.9823

Sweep:

SWEEP1

Files:

/programs/datagrid/361/RG002_6_1_####.cbf

Images:

1 - 450

Beam:

(218.99, 217.73) -> (218.99, 217.56)

Distance:

400.0 -> 398.41

Spacegroup:

P 63 2 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.42 6.56 2.42
Low resolution limit 36.02 36.02 2.46
Completeness 100.0 98.6 100.0
Multiplicity 8.7 5.8 10.2
I/sigma 3.4 3.6 0.7
Rmerge 0.38 0.372 2.364
CC half 0.841 0.735 0.082
Anomalous completeness 98.6 90.0 100.0
Anomalous multiplicity 4.8 4.8 4.8
Cell 106.54 106.54 195.57 90.0 90.0 120.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.9823

Sweep:

SWEEP1

Files:

/programs/datagrid/361/RG002_6_1_####.cbf

Images:

1 - 450

Beam:

(218.99, 217.73) -> (218.99, 219.58)

Distance:

400.0 -> 404.4

Spacegroup:

P 6 2 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.36 6.4 2.36
Low resolution limit 67.18 67.21 2.4
Completeness 99.8 98.6 100.0
Multiplicity 8.7 6.4 10.1
I/sigma 3.5 4.3 0.7
Rmerge 0.378 0.374 2.287
CC half 0.853 0.784 0.109
Anomalous completeness 98.6 91.9 100.0
Anomalous multiplicity 4.7 4.7 4.7
Cell 106.66 106.66 195.76 90.0 90.0 120.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.9823

Sweep:

SWEEP1

Files:

/programs/datagrid/361/RG002_6_1_####.cbf

Images:

1 - 450

Beam:

(218.99, 217.73) -> (218.99, 219.6)

Distance:

400.0 -> 405.36

Spacegroup:

P 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 4.29 11.64 4.29
Low resolution limit 92.81 92.83 4.36
Completeness 53.6 52.7 56.8
Multiplicity 1.6 1.9 1.6
I/sigma 9.4 26.0 5.5
Rmerge 0.161 0.0 0.125
CC half 0.368 0.345 0.089
Anomalous completeness 33.3 45.6 32.7
Anomalous multiplicity 1.0 1.0 1.0
Cell 105.775 106.116 194.724 89.948 89.811 60.105

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.