X-Ray Diffraction data from Human Dpf3 double-PHD domain bound to histone H3 tail peptide with acetylated K14, source of 5SZB structure

Data DOI: 10.15785/SBGRID/358 | ID: 358

5SZB Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Corbett Laboratory, University of California, San Diego

Release Date: Sept. 5, 2017

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/358

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/358 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/358 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/358 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/358 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 358 ; shasum -c files.sha'

Storage requirements: 5.3G

Biological Sample:

Human Dpf3 double-PHD domain bound to histone H3 tail peptide with acetylated K14

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

SSRL Beamline 9-2

Data Creation Date:

June 14, 2016

Related Datasets:

357


Cite this Dataset

Local, A; Ren, B; Shiau, AK; Singh, N; Corbett, KD. 2017. "X-Ray Diffraction data for: Human Dpf3 double-PHD domain bound to histone H3 tail peptide with acetylated K14. PDB Code 5SZB", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/358.

Download Citation

Dataset Description

Native data for PDB 5SZB

Project Members

Name Additional Roles Affiliation While Working on the Project
Kevin D CorbettDepositorUniversity of California, San Diego and Ludwig Institute for Cancer Research
Andrea LocalUniversity of California, San Diego and Ludwig Institute for Cancer Research
Bing RenUniversity of California, San Diego and Ludwig Institute for Cancer Research
Andrew K ShiauLudwig Institute for Cancer Research
Namit SinghData CollectorUniversity of California, San Diego and Ludwig Institute for Cancer Research
Kevin CorbettPIUniversity of California, San Diego

Reprocessing Instructions

Process to 1.2 A with XDS, Space group P4(3)2(1)2

Reprocessing Data

About Reprocessing

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97946

Sweep:

SWEEP1

Files:

/programs/datagrid/358/DPF3_2348_6_#####.cbf

Images:

1 - 900

Beam:

(217.32, 211.82) -> (217.32, 211.74)

Distance:

200.0 -> 199.36

Spacegroup:

P 41 21 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.04 2.82 1.04
Low resolution limit 39.61 39.63 1.06
Completeness 89.7 100.0 23.3
Multiplicity 8.9 10.8 1.8
I/sigma 21.7 45.4 1.4
Rmerge 0.044 0.036 0.443
CC half 0.999 0.998 0.596
Anomalous completeness 79.3 100.0 9.0
Anomalous multiplicity 5.0 5.0 5.0
Cell 39.605 39.605 148.525 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97946

Sweep:

SWEEP1

Files:

/programs/datagrid/358/DPF3_2348_6_#####.cbf

Images:

1 - 900

Beam:

(217.32, 211.82) -> (217.32, 211.43)

Distance:

200.0 -> 199.17

Spacegroup:

P 41 21 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.03 2.8 1.03
Low resolution limit 38.27 38.3 1.05
Completeness 89.8 98.9 17.5
Multiplicity 10.1 11.5 1.7
I/sigma 31.5 72.2 1.0
Rmerge 0.031 0.026 0.568
CC half 1.0 0.999 0.478
Anomalous completeness 79.9 97.5 5.8
Anomalous multiplicity 5.7 5.7 5.7
Cell 39.61 39.61 148.55 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97946

Sweep:

SWEEP1

Files:

/programs/datagrid/358/DPF3_2348_6_#####.cbf

Images:

1 - 900

Beam:

(217.32, 211.82) -> (217.32, 211.43)

Distance:

200.0 -> 199.07

Spacegroup:

P 41 21 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.03 2.8 1.03
Low resolution limit 38.27 38.3 1.05
Completeness 89.8 98.9 17.5
Multiplicity 10.1 11.5 1.7
I/sigma 31.5 72.2 1.0
Rmerge 0.031 0.026 0.561
CC half 1.0 0.999 0.489
Anomalous completeness 79.9 97.5 5.8
Anomalous multiplicity 5.7 5.7 5.7
Cell 39.61 39.61 148.55 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97946

Sweep:

SWEEP1

Files:

/programs/datagrid/358/DPF3_2348_6_#####.cbf

Images:

1 - 900

Beam:

(217.32, 211.82) -> (217.32, 211.43)

Distance:

200.0 -> 199.29

Spacegroup:

P 4 21 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.07 2.9 1.07
Low resolution limit 39.28 39.3 1.09
Completeness 95.5 99.0 60.8
Multiplicity 10.1 11.5 2.8
I/sigma 9.9 19.3 1.2
Rmerge 0.072 0.065 0.488
CC half 0.998 0.998 0.816
Anomalous completeness 86.8 98.7 31.9
Anomalous multiplicity 5.7 5.7 5.7
Cell 39.626 39.626 297.207 90.0 90.0 90.0

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.