X-Ray Diffraction data from Human Dpf3 double-PHD domain bound to histone H3 tail peptide with acetylated K14, source of 5SZB structure

Data DOI: 10.15785/SBGRID/357 | ID: 357

5SZB Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Corbett Laboratory, University of California, San Diego

Release Date: Sept. 5, 2017

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/357

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/357 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/357 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/357 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/357 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 357 ; shasum -c files.sha'

Storage requirements: 5.3G

Biological Sample:

Human Dpf3 double-PHD domain bound to histone H3 tail peptide with acetylated K14

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

SSRL Beamline 9-2

Data Creation Date:

June 14, 2016

Related Datasets:

358


Cite this Dataset

Local, A; Ren, B; Shiau, AK; Singh, N; Corbett, KD. 2017. "X-Ray Diffraction data for: Human Dpf3 double-PHD domain bound to histone H3 tail peptide with acetylated K14. PDB Code 5SZB", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/357.

Download Citation

Dataset Description

Zinc SAD data for PDB entry 5SZB

Project Members

Name Additional Roles Affiliation While Working on the Project
Kevin D CorbettDepositorUniversity of California, San Diego and Ludwig Institute for Cancer Research
Andrea LocalUniversity of California, San Diego and Ludwig Institute for Cancer Research
Bing RenUniversity of California, San Diego and Ludwig Institute for Cancer Research
Andrew K ShiauUniversity of California, San Diego and Ludwig Institute for Cancer Research
Namit SinghData CollectorUniversity of California, San Diego and Ludwig Institute for Cancer Research
Kevin CorbettPIUniversity of California, San Diego

Reprocessing Instructions

Process to 1.55 A with XDS, Spacegroup P4(3)2(1)2

Reprocessing Data

About Reprocessing

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 1.28129

Sweep:

SWEEP1

Files:

/programs/datagrid/357/DPF3_2348-Zn_4_#####.cbf

Images:

1 - 900

Beam:

(217.32, 211.82) -> (217.32, 211.63)

Distance:

200.0 -> 199.3

Spacegroup:

P 41 21 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.36 3.68 1.36
Low resolution limit 49.58 49.61 1.38
Completeness 82.1 100.0 14.1
Multiplicity 9.3 10.0 1.8
I/sigma 25.0 31.5 4.1
Rmerge 0.058 0.054 0.15
CC half 0.998 0.997 0.919
Anomalous completeness 79.0 99.9 6.5
Anomalous multiplicity 5.2 5.2 5.2
Cell 39.598 39.598 148.728 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 1.28129

Sweep:

SWEEP1

Files:

/programs/datagrid/357/DPF3_2348-Zn_4_#####.cbf

Images:

1 - 900

Beam:

(217.32, 211.82) -> (217.32, 211.45)

Distance:

200.0 -> 199.16

Spacegroup:

P 41 21 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.35 3.66 1.35
Low resolution limit 27.1 27.11 1.37
Completeness 83.7 97.8 17.6
Multiplicity 10.7 11.1 2.0
I/sigma 33.4 45.8 4.0
Rmerge 0.047 0.044 0.169
CC half 0.999 0.998 0.899
Anomalous completeness 81.3 94.1 9.1
Anomalous multiplicity 5.9 5.9 5.9
Cell 39.59 39.59 148.7 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 1.28129

Sweep:

SWEEP1

Files:

/programs/datagrid/357/DPF3_2348-Zn_4_#####.cbf

Images:

1 - 900

Beam:

(217.32, 211.82) -> (217.32, 211.45)

Distance:

200.0 -> 199.11

Spacegroup:

P 41 21 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.35 3.66 1.35
Low resolution limit 38.26 38.27 1.37
Completeness 83.7 97.9 17.6
Multiplicity 10.7 11.1 2.0
I/sigma 33.4 45.7 4.0
Rmerge 0.047 0.044 0.17
CC half 0.999 0.998 0.897
Anomalous completeness 81.3 94.6 9.1
Anomalous multiplicity 5.9 5.9 5.9
Cell 39.59 39.59 148.7 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 1.28129

Sweep:

SWEEP1

Files:

/programs/datagrid/357/DPF3_2348-Zn_4_#####.cbf

Images:

1 - 900

Beam:

(217.32, 211.82) -> (217.32, 211.44)

Distance:

200.0 -> 199.57

Spacegroup:

P 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.48 4.02 1.48
Low resolution limit 39.61 39.63 1.51
Completeness 76.8 92.7 31.5
Multiplicity 1.9 1.9 1.8
I/sigma 2.6 3.2 2.1
Rmerge 0.354 0.575 0.391
CC half 0.897 0.884 0.693
Anomalous completeness 50.5 74.1 16.9
Anomalous multiplicity 1.1 1.1 1.1
Cell 39.643 39.649 142.521 87.582 84.977 89.993

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.