Micro-Electron Diffraction data from alpha-synuclein, source of 4RIL structure

Data DOI: 10.15785/SBGRID/223 | ID: 223

Publication DOI: 10.1038/nature15368

4RIL Coordinates: Viewer, PDB, MMDB

Gonen Laboratory, Howard Hughes Medical Institute

Release Date: 4 Mar 2016

Supersedes: 10.15785/SBGRID/193

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/223

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/223 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/223 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/223 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/223 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 223 ; shasum -c files.sha'

Storage requirements: 2.1G

Biological Sample:

alpha-synuclein

Dataset Type:

Micro-Electron Diffraction

Subject Composition:

Protein

Collection Facility:

FEI TF20 (Janelia Research Campus)

Data Creation Date:

25 Aug 2014

Related Datasets:

193


Cite this Dataset

Reyes, FE; Rodriguez, JA; Gonen, T. 2016. "Micro-Electron Diffraction data for: alpha-synuclein. PDB Code 4RIL", SBGrid Data Bank, V1, http://dx.doi.org/10.15785/SBGRID/223.

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Dataset Description

Structure of the amyloid forming segment, GAVVTGVTAVA, from the NAC domain of Parkinson's disease protein alpha-synuclein, residues 68-78, determined by electron diffraction.

Project Members

Name Additional Roles Affiliation While Working on the Project
Francis E ReyesDepositorHHMI Janelia Research Campus
Jose A RodriguezData CollectorHHMI UCLA-DOE Institute
Tamir GonenPIHoward Hughes Medical Institute

Reprocessing Instructions

Convert the images to SMV: $ tvips2smv -d 1900 -r 0.3 -o movie3s1mt_60d_19/movie3s1mt_60d_19_###.img 193/movie3s1mt_60d_19_000.tvips $ tvips2smv -d 1860 -r 0.3 -o movie4s1mt_60d_05/movie4s1mt_60d_05_###.img 193/movie4s1mt_60d_05_000.tvips $ tvips2smv -d 1850 -r 0.3 -o movie4s1mt60d_06/movie4s1mt60d_06_###.img 193/movie4s1mt60d_06_000.tvips $ tvips2smv -d 1860 -r 0.3 -o movie4s1mt_60d_22/movie4s1mt_60d_22_###.img 193/movie4s1mt_60d_22_000.tvips MOSFLM processing parameters (all datasets): ADC offset: 0 Direction of spindle rotation: Forward Gain: 2


License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.