X-Ray Diffraction data from C. elegan PCH-2, source of 4XGU structure

Data DOI: 10.15785/SBGRID/20 | ID: 20

Publication DOI: 10.7554/eLife.07367

4XGU Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Corbett Laboratory, University of California, San Diego

Release Date: 6 May 2015

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/20

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/20 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/20 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/20 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/20 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 20 ; shasum -c files.sha'

Storage requirements: 11G

Biological Sample:

C. elegan PCH-2

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

SSRL Beamline 12-2

Data Creation Date:

3 Mar 2014

Related Datasets:

21


Cite this Dataset

Ye, Q; Corbett, KD. 2015. "X-Ray Diffraction data for: C. elegan PCH-2. PDB Code 4XGU", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/20.

Download Citation

Dataset Description

Native dataset of C. elegans PCH-2, crystallized without addition of nucleotides

Project Members

Name Additional Roles Affiliation While Working on the Project
Qiaozhen YeData CollectorUniversity of California, San Diego
Kevin D CorbettDepositor, PIUniversity of California, San Diego

Reprocessing Instructions

Process with XDS to 2.3 Å, perform anisotropic data cutoffs at 2.3 Å for a* and b*, and 3.2 Å on c*, and apply anisotropic scaling B-factors of -8.09 (a*), -8.02 (b*), 16.11 (c*) followed by isotropic b-factor correction of -19.65 (originally done automatically with UCLA diffraction anisotropy server)

Reprocessing Data

About Reprocessing

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.9795

Sweep:

SWEEP1

Files:

/programs/datagrid/20/mD5_2_#####.cbf

Images:

1 - 1800

Beam:

(217.32, 211.82) -> (217.32, 211.87)

Distance:

400.0 -> 399.95

Spacegroup:

C 2 2 21

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.37 6.43 2.37
Low resolution limit 120.4 120.55 2.41
Completeness 99.6 99.5 99.6
Multiplicity 11.1 11.7 10.7
I/sigma 10.2 27.7 1.0
Rmerge 0.12 0.052 2.915
CC half 0.998 0.999 0.363
Anomalous completeness 99.4 99.7 98.2
Anomalous multiplicity 5.7 5.7 5.7
Cell 126.462 240.796 197.644 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.9795

Sweep:

SWEEP1

Files:

/programs/datagrid/20/mD5_2_#####.cbf

Images:

1 - 1800

Beam:

(217.32, 211.82) -> (217.32, 211.76)

Distance:

400.0 -> 400.7

Spacegroup:

C 2 2 21

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.93 7.94 2.93
Low resolution limit 42.64 42.64 2.98
Completeness 99.7 99.4 99.8
Multiplicity 13.4 12.0 14.2
I/sigma 33.1 70.4 6.6
Rmerge 0.049 0.024 0.469
CC half 1.0 1.0 0.974
Anomalous completeness 99.7 99.1 99.8
Anomalous multiplicity 7.0 7.0 7.0
Cell 126.72 241.02 197.93 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.9795

Sweep:

SWEEP1

Files:

/programs/datagrid/20/mD5_2_#####.cbf

Images:

1 - 1800

Beam:

(217.32, 211.82) -> (217.32, 211.76)

Distance:

400.0 -> 400.01

Spacegroup:

C 2 2 21

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.49 6.75 2.49
Low resolution limit 45.7 45.71 2.53
Completeness 99.9 99.4 99.9
Multiplicity 13.4 12.4 13.9
I/sigma 21.6 66.6 1.5
Rmerge 0.067 0.025 2.117
CC half 1.0 1.0 0.742
Anomalous completeness 99.9 99.1 99.8
Anomalous multiplicity 6.9 6.9 6.9
Cell 126.72 241.02 197.93 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.9795

Sweep:

SWEEP1

Files:

/programs/datagrid/20/mD5_2_#####.cbf

Images:

1 - 1800

Beam:

(217.32, 211.82) -> (217.32, 211.76)

Distance:

400.0 -> 399.91

Spacegroup:

C 2 2 21

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.37 6.43 2.37
Low resolution limit 74.15 74.18 2.41
Completeness 99.9 100.0 99.4
Multiplicity 13.2 12.8 13.7
I/sigma 15.8 48.9 1.1
Rmerge 0.078 0.041 2.179
CC half 0.999 0.999 0.74
Anomalous completeness 99.9 99.8 99.4
Anomalous multiplicity 6.8 6.8 6.8
Cell 126.624 240.819 197.761 90.0 90.0 90.0

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.