X-Ray Diffraction data from Nup37-Nup120(aa1-961) complex from Schizosaccharomyces pombe, source of 4FHM structure

Data DOI: 10.15785/SBGRID/182 | ID: 182

Publication DOI: 10.1073/pnas.1205151109

4FHM Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Schwartz Laboratory, Massachusetts Institute of Technology

Release Date: 6 Oct 2015

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/182

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/182 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/182 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/182 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/182 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 182 ; shasum -c files.sha'

Storage requirements: 3.2G

Biological Sample:

Nup37-Nup120(aa1-961) complex from Schizosaccharomyces pombe

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

APS 24 ID-C

Data Creation Date:

10 Dec 2010

Related Datasets:

181


Cite this Dataset

Bilokapic, S; Schwartz, TU. 2015. "X-Ray Diffraction data for: Nup37-Nup120(aa1-961) complex from Schizosaccharomyces pombe. PDB Code 4FHM", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/182.

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Dataset Description

SeMet data, collected on two separate spots on a single crystal

Project Members

Name Additional Roles Affiliation While Working on the Project
Thomas U SchwartzData Collector, DepositorMIT
Silvija BilokapicData CollectorMIT
Thomas SchwartzPIMassachusetts Institute of Technology

Reprocessing Instructions

x beam 156.1 y beam 155.7

Reprocessing Data

About Reprocessing

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97911

Sweep:

SWEEP2

Files:

/programs/datagrid/182/p613se_2_###.img

Images:

1 - 90

Beam:

(155.51, 156.23) -> (155.51, 155.97)

Distance:

780.0 -> 788.31

Spacegroup:

P 41 21 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 4.74 12.85 4.74
Low resolution limit 79.32 79.33 4.82
Completeness 99.8 100.0 95.6
Multiplicity 12.5 11.9 4.6
I/sigma 10.3 37.3 1.0
Rmerge 0.124 0.048 1.355
CC half 0.998 0.997 0.292
Anomalous completeness 99.3 99.6 92.5
Anomalous multiplicity 6.8 6.8 6.8
Cell 164.96 164.96 317.29 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97911

Sweep:

SWEEP2

Files:

/programs/datagrid/182/p613se_2_###.img

Images:

1 - 90

Beam:

(155.51, 156.23) -> (155.51, 155.98)

Distance:

780.0 -> 785.77

Spacegroup:

P 41 21 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 4.67 12.67 4.67
Low resolution limit 164.99 165.09 4.75
Completeness 99.5 100.0 93.3
Multiplicity 12.2 11.9 4.4
I/sigma 10.0 37.0 0.9
Rmerge 0.125 0.049 1.558
CC half 0.998 0.997 0.26
Anomalous completeness 98.8 99.7 89.0
Anomalous multiplicity 6.6 6.6 6.6
Cell 164.99 164.99 317.55 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97911

Sweep:

SWEEP2

Files:

/programs/datagrid/182/p613se_2_###.img

Images:

1 - 90

Beam:

(155.51, 156.23) -> (155.51, 155.98)

Distance:

780.0 -> 844.33

Spacegroup:

P 41 21 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 4.61 12.52 4.61
Low resolution limit 124.52 124.57 4.69
Completeness 98.2 100.0 60.6
Multiplicity 12.2 12.6 4.9
I/sigma 6.3 46.1 0.1
Rmerge 0.154 0.054 4.566
CC half 0.996 0.997 0.272
Anomalous completeness 97.5 100.0 57.6
Anomalous multiplicity 6.6 6.6 6.6
Cell 176.105 176.105 345.031 90.0 90.0 90.0

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.