X-Ray Diffraction data from Nup37-Nup120(aa1-961) complex from Schizosaccharomyces pombe, source of 4FHM structure

Data DOI: 10.15785/SBGRID/181 | ID: 181

Publication DOI: 10.1073/pnas.1205151109

4FHM Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Schwartz Laboratory, Massachusetts Institute of Technology

Release Date: Oct. 6, 2015

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/181

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/181 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/181 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/181 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/181 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 181 ; shasum -c files.sha'

Storage requirements: 3.2G

Biological Sample:

Nup37-Nup120(aa1-961) complex from Schizosaccharomyces pombe

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

APS 24 ID-C

Data Creation Date:

Oct. 17, 2010

Related Datasets:

182


Cite this Dataset

Bilokapic, S; Schwartz, TU. 2015. "X-Ray Diffraction data for: Nup37-Nup120(aa1-961) complex from Schizosaccharomyces pombe. PDB Code 4FHM", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/181.

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Dataset Description

native data, collected on four spots on the same crystal

Project Members

Name Additional Roles Affiliation While Working on the Project
Thomas U SchwartzData Collector, DepositorMIT
Silvija BilokapicData CollectorMIT
Thomas SchwartzPIMassachusetts Institute of Technology

Reprocessing Instructions

x beam 156.6 y beam 156.7

Reprocessing Data

About Reprocessing

Version:

version unreported

Reprocessing failed.

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97918

Sweep:

SWEEP4

Files:

/programs/datagrid/181/p_315_4_###.img

Images:

1 - 45

Beam:

(156.16, 156.26) -> (156.16, 156.01)

Distance:

625.0 -> 630.88

Spacegroup:

P 41 21 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 4.8 13.02 4.8
Low resolution limit 109.34 109.37 4.88
Completeness 99.7 100.0 99.3
Multiplicity 6.4 11.8 3.7
I/sigma 9.3 33.3 1.6
Rmerge 0.085 0.057 0.673
CC half 0.998 0.998 0.64
Anomalous completeness 95.7 99.6 92.5
Anomalous multiplicity 3.5 3.5 3.5
Cell 165.12 165.12 311.81 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97918

Sweep:

SWEEP4

Files:

/programs/datagrid/181/p_315_4_###.img

Images:

1 - 45

Beam:

(156.16, 156.26) -> (156.16, 156.0)

Distance:

625.0 -> 632.31

Spacegroup:

P 41 21 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 4.87 13.2 4.87
Low resolution limit 77.96 77.97 4.95
Completeness 100.0 100.0 99.4
Multiplicity 8.9 11.7 3.7
I/sigma 10.2 34.1 1.7
Rmerge 0.102 0.057 0.593
CC half 0.998 0.998 0.67
Anomalous completeness 99.0 99.6 92.4
Anomalous multiplicity 4.9 4.9 4.9
Cell 165.13 165.13 311.83 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97918

Sweep:

SWEEP4

Files:

/programs/datagrid/181/p_315_4_###.img

Images:

1 - 45

Beam:

(156.16, 156.26) -> (156.16, 155.98)

Distance:

625.0 -> 620.57

Spacegroup:

P 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 4.84 13.14 4.84
Low resolution limit 110.62 110.65 4.93
Completeness 68.2 87.8 15.8
Multiplicity 1.9 2.2 1.4
I/sigma 4.3 10.0 1.3
Rmerge 0.073 0.044 0.0
CC half 0.997 0.998 0.656
Anomalous completeness 45.3 76.7 6.8
Anomalous multiplicity 1.1 1.1 1.1
Cell 166.048 167.196 314.833 89.794 90.271 89.705

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.