X-Ray Diffraction data from HSV-1 glycoprotein B, source of 2GUM structure

Data DOI: 10.15785/SBGRID/122 | ID: 122

Publication DOI: 10.1126/science.1126548

2GUM Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Heldwein Laboratory, Tufts University School of Medicine

Release Date: May 21, 2015

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/122

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/122 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/122 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/122 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/122 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 122 ; shasum -c files.sha'

Storage requirements: 7.2G

Biological Sample:

HSV-1 glycoprotein B

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

APS 24-ID-C

Data Creation Date:

Aug. 19, 2005

Related Datasets:

120


Cite this Dataset

Heldwein, EE. 2015. "X-Ray Diffraction data for: HSV-1 glycoprotein B. PDB Code 2GUM", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/122.

Download Citation

Dataset Description

This is a selenomethionine single-wavelength anomalous diffraction data set collected at 0.97950 angstrom using inverse beam strategy

Project Members

Name Additional Roles Affiliation While Working on the Project
Ekaterina E HeldweinData Collector, DepositorChildren's Hospital Boston/Harvard Medical School
Ekaterina E HeldweinPITufts University School of Medicine

Reprocessing Instructions

none

Reprocessing Data

About Reprocessing

Version:

version unreported

Reprocessing failed.

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.9795

Sweep:

SWEEP2

Files:

/programs/datagrid/122/tryp_smet_24_peak_101_###.img

Images:

1 - 190

Beam:

(156.09, 156.4) -> (156.09, 155.87)

Distance:

400.0 -> 400.6

Spacegroup:

P 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.72 7.37 2.72
Low resolution limit 48.33 48.33 2.77
Completeness 98.3 99.2 97.3
Multiplicity 4.1 4.1 2.8
I/sigma 8.7 22.7 1.0
Rmerge 0.093 0.044 1.046
CC half 0.973 0.941 0.441
Anomalous completeness 97.1 97.4 89.6
Anomalous multiplicity 2.1 2.1 2.1
Cell 85.43 100.25 100.36 67.22 69.98 78.08

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.9795

Sweep:

SWEEP2

Files:

/programs/datagrid/122/tryp_smet_24_peak_101_###.img

Images:

1 - 190

Beam:

(156.09, 156.4) -> (156.09, 155.87)

Distance:

400.0 -> 400.54

Spacegroup:

P 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.73 7.41 2.73
Low resolution limit 68.67 68.69 2.78
Completeness 98.2 99.1 97.0
Multiplicity 4.1 4.1 2.4
I/sigma 8.8 22.7 1.0
Rmerge 0.091 0.044 1.193
CC half 0.994 0.993 0.419
Anomalous completeness 96.8 97.2 87.0
Anomalous multiplicity 2.0 2.0 2.0
Cell 85.43 100.25 100.36 67.22 69.98 78.08

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.9795

Sweep:

SWEEP2

Files:

/programs/datagrid/122/tryp_smet_24_peak_101_###.img

Images:

1 - 190

Beam:

(156.09, 156.4) -> (156.09, 155.85)

Distance:

400.0 -> 400.69

Spacegroup:

P 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.68 7.27 2.68
Low resolution limit 68.65 68.67 2.72
Completeness 98.7 99.8 94.1
Multiplicity 4.1 4.1 3.2
I/sigma 7.7 18.4 1.2
Rmerge 0.096 0.052 0.844
CC half 0.988 0.98 0.696
Anomalous completeness 97.6 99.3 88.6
Anomalous multiplicity 2.1 2.1 2.1
Cell 85.391 100.249 100.376 67.23 70.003 78.082

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.