X-Ray Diffraction data from HSV-1 glycoprotein B, source of 2GUM structure

Data DOI: 10.15785/SBGRID/120 | ID: 120

Publication DOI: 10.1126/science.1126548

2GUM Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Heldwein Laboratory, Tufts University School of Medicine

Release Date: May 21, 2015

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/120

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/120 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/120 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/120 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/120 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 120 ; shasum -c files.sha'

Storage requirements: 17G

Biological Sample:

HSV-1 glycoprotein B

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

APS BEAMLINE 24-ID-C

Data Creation Date:

June 16, 2005

Related Datasets:

122


Cite this Dataset

Heldwein, EE. 2015. "X-Ray Diffraction data for: HSV-1 glycoprotein B. PDB Code 2GUM", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/120.

Download Citation

Dataset Description

Native data set collected at 0.97944 angstrom

Project Members

Name Additional Roles Affiliation While Working on the Project
Ekaterina E HeldweinData Collector, DepositorChildren's Hospital Boston/Harvard Medical School
Ekaterina E HeldweinPITufts University School of Medicine

Reprocessing Instructions

This data set was collected from a crystal that diffracted x-rays to 2.1 angstrom

Reprocessing Data

About Reprocessing

Version:

version unreported

Reprocessing failed.

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.9498

Sweep:

SWEEP2

Files:

/programs/datagrid/120/native_kh_1_2_2_###.img

Images:

1 - 400

Beam:

(158.56, 148.42) -> (158.56, 148.39)

Distance:

299.99 -> 300.67

Spacegroup:

P 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.95 5.29 1.95
Low resolution limit 77.21 77.27 1.98
Completeness 97.3 99.4 83.3
Multiplicity 5.1 4.9 3.7
I/sigma 11.6 32.5 1.2
Rmerge 0.06 0.037 1.072
CC half 0.971 0.922 0.652
Anomalous completeness 95.8 97.8 73.9
Anomalous multiplicity 2.6 2.6 2.6
Cell 82.32 96.96 97.09 67.06 78.04 70.32

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.9498

Sweep:

SWEEP2

Files:

/programs/datagrid/120/native_kh_1_2_2_###.img

Images:

1 - 400

Beam:

(158.56, 148.42) -> (158.56, 148.39)

Distance:

299.99 -> 300.85

Spacegroup:

P 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.93 5.24 1.93
Low resolution limit 77.21 77.28 1.96
Completeness 96.6 99.4 75.7
Multiplicity 5.1 4.9 3.1
I/sigma 11.3 32.3 1.0
Rmerge 0.06 0.038 1.196
CC half 0.961 0.898 0.563
Anomalous completeness 94.8 97.8 65.5
Anomalous multiplicity 2.6 2.6 2.6
Cell 82.32 96.96 97.09 67.06 78.04 70.32

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.9498

Sweep:

SWEEP2

Files:

/programs/datagrid/120/native_kh_1_2_2_###.img

Images:

1 - 400

Beam:

(158.56, 148.42) -> (158.56, 140.46)

Distance:

299.99 -> 305.72

Spacegroup:

P 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.06 5.6 2.06
Low resolution limit 85.74 85.82 2.1
Completeness 96.6 97.7 73.5
Multiplicity 3.6 4.7 3.0
I/sigma 2.3 4.6 0.5
Rmerge 0.408 0.594 0.626
CC half 0.882 0.524 0.869
Anomalous completeness 93.2 93.6 71.2
Anomalous multiplicity 1.8 1.8 1.8
Cell 82.436 96.997 97.163 66.954 78.105 70.681

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.