Two raw tilt-series (in MRC format) of FIB-milled G-TPP-treated HeLa cells that have been milled. The dataset contains mitochondria and cytoplasmic ribosomes as well as mHSP60-mHSP10 chaperonin "football" complexes. MRC files and MDOC files are provided. See the Processing Bundle below for all the scripts necessary to reconstruct the data to low-resolution following our tutorial. This tutorial involves template matching, subtomogram averaging, membrane segmentation, and particle place-back. See the processing instruction document for full details. Storage required: 4.4 G (dataset size) and 50G (storage for processing files when the tutorial has been completed).
Data DOI: 10.15785/SBGRID/1273 | Publication DOI: 10.1101/2025.10.06.680693 | Published: 10 Jul 2026
Ruben Fernandez-Busnadiego, University of Göttingen
