Data DOI: 10.15785/SBGRID/1273 | ID: 1273
Publication DOI: 10.1101/2025.10.06.680693
Ruben Fernandez-Busnadiego, University of Göttingen
Release Date: 10 Jul 2026
1. If this dataset is locally available, it should be accessable at /programs/datagrid/1273
2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:
'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/1273 .' (Harvard Medical School, USA)
Depending on your location, faster access may be available from a Tier 1 site closer to your location
'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/1273 .' (Uppsala University, Sweden)
'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/1273 .' (Institut Pasteur de Montevideo, Uruguay)
'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/1273 .' (Shanghai Institutes for Biological Sciences, China)
3. After the transfer is completed, please issue the following command to verify data integrity:
'cd 1273 ; shasum -c files.sha'
Storage requirements: 4.4G
Biological Sample:
CryoET Training data from milled G-TPP-treated HeLa cells (tilt-series)
Dataset Type:
CryoET Training
Subject Composition:
Protein
Collection Facility:
Unknown
Data Creation Date:
19 Oct 2022
Related Datasets:
None
Eruera, A; Antico, O; Azem, A; Ehses, K; Fernández-Busnadiego, R; Lopez-Alonso, JP; Muqit, MM. K; Ubarretxena-Belandia, I; Ruben Fernandez-Busnadiego, R. 2026. "CryoET Training data for: CryoET Training data from milled G-TPP-treated HeLa cells (tilt-series).", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/1273.
Two raw tilt-series (in MRC format) of FIB-milled G-TPP-treated HeLa cells that have been milled. The dataset contains mitochondria and cytoplasmic ribosomes as well as mHSP60-mHSP10 chaperonin "football" complexes. MRC files and MDOC files are provided. See the Processing Bundle below for all the scripts necessary to reconstruct the data to low-resolution following our tutorial. This tutorial involves template matching, subtomogram averaging, membrane segmentation, and particle place-back. See the processing instruction document for full details. Storage required: 4.4 G (dataset size) and 50G (storage for processing files when the tutorial has been completed).
| Name | Additional Roles | Affiliation While Working on the Project |
|---|---|---|
| Alice-Roza Eruera | Depositor | Harvard Medical School |
| Odetta Antico | Data Collector | Aligning Science Across Parkinson’s (ASAP) Collaborative Research Network, |
| Abdussalam Azem | Data Collector | Tel Aviv University |
| Kenneth Ehses | Data Collector | University Medical Center Göttingen, Institute for Neuropathology, Göttingen, Germany |
| Rubén Fernández-Busnadiego | Data Collector | University Medical Center Göttingen |
| Jorge P Lopez-Alonso | Data Collector | Biofisika Institute (CSIC-UPV/EHU), University of the Basque Country, Leioa, Spain |
| Miratul M. K Muqit | Data Collector | Aligning Science Across Parkinson’s (ASAP) Collaborative Research Network |
| Iban Ubarretxena-Belandia | Data Collector | Biofisika Institute (CSIC-UPV/EHU), University of the Basque Country, Leioa, Spain. |
| Ruben Ruben Fernandez-Busnadiego | PI | University of Göttingen |
The processing instructions can be found here: https://docs.google.com/document/d/1heC_HsmRq3WPnsQAt1TAbMOwjDI-gQnDtwUeipbs3gQ/edit?tab=t.0 Please download the Processing Bundle attached to obtain the scripts referenced in the documentation above.
License: CC0
Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.