Data DOI: 10.15785/SBGRID/1273 | ID: 1273
Publication DOI: 10.1101/2025.10.06.680693
Ruben Fernandez-Busnadiego, University of Göttingen
Release Date: 10 Jul 2026
Two raw tilt-series (in MRC format) of FIB-milled G-TPP-treated HeLa cells that have been milled. The dataset contains mitochondria and cytoplasmic ribosomes as well as mHSP60-mHSP10 chaperonin "football" complexes. MRC files and MDOC files are provided. See the Processing Bundle below for all the scripts necessary to reconstruct the data to low-resolution following our tutorial. This tutorial involves template matching, subtomogram averaging, membrane segmentation, and particle place-back. See the processing instruction document for full details. Storage required: 4.4 G (dataset size) and 50G (storage for processing files when the tutorial has been completed).
The processing instructions can be found in our data processing guide. Please download the Processing Bundle attached to obtain the scripts referenced in the documentation above.
1. If this dataset is locally available, it should be accessable at /programs/datagrid/1273
2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:
'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/1273 .' (Harvard Medical School, USA)
Depending on your location, faster access may be available from a Tier 1 site closer to your location
'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/1273 .' (Uppsala University, Sweden)
'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/1273 .' (Institut Pasteur de Montevideo, Uruguay)
'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/1273 .' (Shanghai Institutes for Biological Sciences, China)
3. After the transfer is completed, please issue the following command to verify data integrity:
'cd 1273 ; shasum -c files.sha'
Storage requirements: 4.4G
Biological Sample:
CryoET Training data from milled G-TPP-treated HeLa cells (tilt-series)
Dataset Type:
CryoET Training
Subject Composition:
Protein
Collection Facility:
Unknown
Data Creation Date:
19 Oct 2022
Related Datasets:
None
Eruera, A; Antico, O; Azem, A; Ehses, K; Fernández-Busnadiego, R; Lopez-Alonso, JP; Muqit, MM. K; Ubarretxena-Belandia, I; Ruben Fernandez-Busnadiego, R. 2026. "CryoET Training data for: CryoET Training data from milled G-TPP-treated HeLa cells (tilt-series).", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/1273.
| Name | Additional Roles | Affiliation While Working on the Project |
|---|---|---|
| Alice-Roza Eruera | Depositor | Harvard Medical School |
| Odetta Antico | Data Collector | Aligning Science Across Parkinson’s (ASAP) Collaborative Research Network, |
| Abdussalam Azem | Data Collector | Tel Aviv University |
| Kenneth Ehses | Data Collector | University Medical Center Göttingen, Institute for Neuropathology, Göttingen, Germany |
| Rubén Fernández-Busnadiego | Data Collector | University Medical Center Göttingen |
| Jorge P Lopez-Alonso | Data Collector | Biofisika Institute (CSIC-UPV/EHU), University of the Basque Country, Leioa, Spain |
| Miratul M. K Muqit | Data Collector | Aligning Science Across Parkinson’s (ASAP) Collaborative Research Network |
| Iban Ubarretxena-Belandia | Data Collector | Biofisika Institute (CSIC-UPV/EHU), University of the Basque Country, Leioa, Spain. |
| Ruben Ruben Fernandez-Busnadiego | PI | University of Göttingen |
License: CC0
Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.