X-Ray Diffraction data from C5a-peptidase (ScpA) D783A mutant, source of 7YZX structure

Data DOI: 10.15785/SBGRID/960 | ID: 960

Publication DOI: Not yet available

7YZX Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Cooney Laboratory, University of Limerick

Release Date: Sept. 9, 2022

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/960

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/960 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/960 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/960 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/960 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 960 ; shasum -c files.sha'

Storage requirements: 7.6G

Biological Sample:

C5a-peptidase (ScpA) D783A mutant

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

Diamond Light Source, beamline I24.

Data Creation Date:

Feb. 20, 2020

Related Datasets:

None


Cite this Dataset

Kagawa, TF.; Cooney, JC.. 2022. "X-Ray Diffraction data for: C5a-peptidase (ScpA) D783A mutant. PDB Code 7YZX", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/960.

Download Citation

Dataset Description

Native dataset.

Project Members

Name Additional Roles Affiliation While Working on the Project
Todd F. KagawaData Collector, DepositorUniversity of Limerick
Jakki C. CooneyData Collector, PIUniversity of Limerick

Reprocessing Instructions

DECTRIS PILATUS 6M detector. 1300 frames processed with XDS to 1.9 angstrom resolution. Data indexed in space group P6333.

Reprocessing Data

About Reprocessing

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.96864

Sweep:

SWEEP1

Files:

/programs/datagrid/960/783-1_1_####.cbf

Images:

1 - 1300

Beam:

(224.81, 216.99) -> (224.81, 216.89)

Distance:

331.61 -> 330.82

Spacegroup:

P 63 2 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.0 5.43 2.0
Low resolution limit 101.86 102.0 2.03
Completeness 100.0 99.1 100.0
Multiplicity 13.8 12.2 14.2
I/sigma 12.8 40.3 1.1
Rmerge 0.131 0.044 2.91
CC half 0.998 0.999 0.331
Anomalous completeness 99.8 99.5 99.9
Anomalous multiplicity 7.3 7.3 7.3
Cell 169.08 169.08 141.8 90.0 90.0 120.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.96864

Sweep:

SWEEP1

Files:

/programs/datagrid/960/783-1_1_####.cbf

Images:

1 - 1300

Beam:

(224.81, 216.99) -> (224.81, 216.88)

Distance:

331.61 -> 331.67

Spacegroup:

P 63 2 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.99 5.4 1.99
Low resolution limit 101.87 102.0 2.02
Completeness 100.0 99.1 100.0
Multiplicity 13.8 12.2 14.2
I/sigma 12.7 40.3 1.1
Rmerge 0.131 0.044 2.988
CC half 0.998 0.998 0.302
Anomalous completeness 99.8 99.6 99.9
Anomalous multiplicity 7.3 7.3 7.3
Cell 169.09 169.09 141.81 90.0 90.0 120.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.96864

Sweep:

SWEEP1

Files:

/programs/datagrid/960/783-1_1_####.cbf

Images:

1 - 1300

Beam:

(224.81, 216.99) -> (224.81, 216.88)

Distance:

331.61 -> 332.17

Spacegroup:

P 63 2 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.9 5.15 1.9
Low resolution limit 141.57 141.97 1.93
Completeness 100.0 99.5 99.6
Multiplicity 13.9 12.7 14.3
I/sigma 5.6 34.8 0.1
Rmerge 0.211 0.061 5.618
CC half 0.994 0.997 0.117
Anomalous completeness 99.9 100.0 99.6
Anomalous multiplicity 7.3 7.3 7.3
Cell 168.617 168.617 141.571 90.0 90.0 120.0

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.