X-Ray Diffraction data from AS4.3-RNASEH2b-HLA*B27, source of 7N2P structure

Data DOI: 10.15785/SBGRID/917 | ID: 917

Publication DOI: 10.1038/s41586-022-05501-7

7N2P Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Garcia Laboratory, Stanford University

Release Date: 9 Dec 2022

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/917

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/917 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/917 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/917 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/917 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 917 ; shasum -c files.sha'

Storage requirements: 5.3G

Biological Sample:

AS4.3-RNASEH2b-HLA*B27

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

ALS 2.0.1

Data Creation Date:

1 Sep 2022

Related Datasets:

None


Cite this Dataset

Jude, KM; Garcia, KC; Yang, X; Garcia, K. 2022. "X-Ray Diffraction data for: AS4.3-RNASEH2b-HLA*B27. PDB Code 7N2P", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/917.

Download Citation

Dataset Description

native dataset

Project Members

Name Additional Roles Affiliation While Working on the Project
Kevin M JudeData Collector, DepositorHHMI/Stanford University
K C GarciaHHMI/Stanford University
Xinbo YangStanford University
K. Christopher GarciaPIStanford University

Reprocessing Instructions

The crystal to detector distance in the image header is incorrect. The correct distance is 390 mm. The first 480 frames were used for structure solution.

Reprocessing Data

About Reprocessing

Version:

version unreported

Reprocessing failed.

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 1.04601

Sweep:

SWEEP1

Files:

/programs/datagrid/917/E12_30_#####.cbf

Images:

1 - 900

Beam:

(219.47, 204.51) -> (219.47, 204.79)

Distance:

250.0 -> 279.27

Spacegroup:

P 2 2 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.29 3.49 1.29
Low resolution limit 27.09 27.09 1.31
Completeness 42.7 99.4 0.1
Multiplicity 4.0 7.5 1.0
I/sigma 0.0 0.0 0.1
Rmerge 4.609 5.216 0.0
CC half 0.076 0.382 0.0
Anomalous completeness 28.0 99.0 0.0
Anomalous multiplicity 2.5 2.5 2.5
Cell 66.61 109.36 198.94 90.0 90.0 90.0

Version:

version unreported

Reprocessing failed.


License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.