X-Ray Diffraction data from Bacteriophytochrome Response Regulator RtBrr, source of 5IC5 structure

Data DOI: 10.15785/SBGRID/903 | ID: 903

Publication DOI: 10.1128/JB.00872-15

5IC5 Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Forest Laboratory, University of Wisconsin-Madison

Release Date: April 15, 2022

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/903

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/903 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/903 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/903 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/903 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 903 ; shasum -c files.sha'

Storage requirements: 12G

Biological Sample:

Bacteriophytochrome Response Regulator RtBrr

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

Advanced Photon Source, LS-Cat, 21-ID_D

Data Creation Date:

Oct. 10, 2013

Related Datasets:

None


Cite this Dataset

Satyshur, KA; Baker, AW; Forest, KT. 2022. "X-Ray Diffraction data for: Bacteriophytochrome Response Regulator RtBrr. PDB Code 5IC5", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/903.

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Dataset Description

720 frames were collected at 1 degree/frame but only 360 were used. Contains both Zn and Cacodylate molecules (As) and has an anomalous signal.

Project Members

Name Additional Roles Affiliation While Working on the Project
Kenneth A SatyshurData Collector, DepositorSinor Scientist
Anna W BakerData CollectorGraduate Student
Katrina T ForestPIUniversity of Wisconsin-Madison

Reprocessing Instructions

Keep Friedel mates separate since there is an anomalous signal. The wavelength is at 0.97910 so both Zn and As have a signal. Contains a unique protein Metal Organic Framework structure on the C-terminal His tag.


License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.