X-Ray Diffraction data from Bacteriophytochrome Response Regulator RtBrr, source of 5IC5 structure

Data DOI: 10.15785/SBGRID/903 | ID: 903

Publication DOI: 10.1128/JB.00872-15

5IC5 Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Forest Laboratory, University of Wisconsin-Madison

Release Date: April 15, 2022

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/903

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/903 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/903 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/903 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/903 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 903 ; shasum -c files.sha'

Storage requirements: 12G

Biological Sample:

Bacteriophytochrome Response Regulator RtBrr

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

Advanced Photon Source, LS-Cat, 21-ID_D

Data Creation Date:

Oct. 10, 2013

Related Datasets:

None


Cite this Dataset

Satyshur, KA; Baker, AW; Forest, KT. 2022. "X-Ray Diffraction data for: Bacteriophytochrome Response Regulator RtBrr. PDB Code 5IC5", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/903.

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Dataset Description

720 frames were collected at 1 degree/frame but only 360 were used. Contains both Zn and Cacodylate molecules (As) and has an anomalous signal.

Project Members

Name Additional Roles Affiliation While Working on the Project
Kenneth A SatyshurData Collector, DepositorSinor Scientist
Anna W BakerData CollectorGraduate Student
Katrina T ForestPIUniversity of Wisconsin-Madison

Reprocessing Instructions

Keep Friedel mates separate since there is an anomalous signal. The wavelength is at 0.97910 so both Zn and As have a signal. Contains a unique protein Metal Organic Framework structure on the C-terminal His tag.

Reprocessing Data

About Reprocessing

Version:

version unreported

Reprocessing failed.

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.9791

Sweep:

SWEEP1

Files:

/programs/datagrid/903/AB3_4_no2.###

Images:

1 - 360

Beam:

(150.0, 150.0) -> (150.0, 149.88)

Distance:

250.0 -> 249.12

Spacegroup:

P 41 2 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.8 4.88 1.8
Low resolution limit 38.34 38.35 1.83
Completeness 100.0 100.0 100.0
Multiplicity 27.2 21.8 23.8
I/sigma 18.6 53.5 0.7
Rmerge 0.111 0.046 5.349
CC half 0.999 0.999 0.317
Anomalous completeness 100.0 100.0 99.9
Anomalous multiplicity 15.0 15.0 15.0
Cell 47.7 47.7 193.37 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.9791

Sweep:

SWEEP1

Files:

/programs/datagrid/903/AB3_4_no2.###

Images:

1 - 360

Beam:

(150.0, 150.0) -> (150.0, 149.89)

Distance:

250.0 -> 249.77

Spacegroup:

P 41 2 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.8 4.88 1.8
Low resolution limit 38.34 38.35 1.83
Completeness 100.0 100.0 100.0
Multiplicity 27.2 21.8 23.8
I/sigma 18.6 53.5 0.7
Rmerge 0.111 0.046 5.346
CC half 0.999 0.999 0.328
Anomalous completeness 100.0 100.0 99.9
Anomalous multiplicity 15.0 15.0 15.0
Cell 47.7 47.7 193.37 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.9791

Sweep:

SWEEP1

Files:

/programs/datagrid/903/AB3_4_no2.###

Images:

1 - 360

Beam:

(150.0, 150.0) -> (150.0, 149.97)

Distance:

250.0 -> 249.15

Spacegroup:

P 41 2 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.78 4.83 1.78
Low resolution limit 48.26 48.27 1.81
Completeness 100.0 100.0 100.0
Multiplicity 27.5 24.0 20.3
I/sigma 12.4 71.2 0.1
Rmerge 0.124 0.056 -21.605
CC half 0.999 0.999 0.306
Anomalous completeness 100.0 100.0 100.0
Anomalous multiplicity 15.2 15.2 15.2
Cell 47.759 47.759 193.579 90.0 90.0 90.0

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.