X-Ray Diffraction data from horse liver alcohol dehydrogenase G173A with NAD and pentafluorobenzyl alcohol at 1.2 A , 50 K, source of 7UHV structure

Data DOI: 10.15785/SBGRID/899 | ID: 899

7UHV Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Plapp Laboratory, University of Iowa

Release Date: April 5, 2022

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/899

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/899 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/899 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/899 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/899 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 899 ; shasum -c files.sha'

Storage requirements: 14G

Biological Sample:

horse liver alcohol dehydrogenase G173A with NAD and pentafluorobenzyl alcohol at 1.2 A , 50 K

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

APS19-ID

Data Creation Date:

June 24, 2009

Related Datasets:

None


Cite this Dataset

Plapp, BV; Plapp, B. 2022. "X-Ray Diffraction data for: horse liver alcohol dehydrogenase G173A with NAD and pentafluorobenzyl alcohol at 1.2 A , 50 K. PDB Code 7UHV", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/899.

Download Citation

Dataset Description

two passes, phi = 0 and 180 60 K with He cryostat

Project Members

Name Additional Roles Affiliation While Working on the Project
B V PlappData Collector, DepositorThe University of Iowa
Bryce PlappPIUniversity of Iowa

Reprocessing Instructions

beam center 1577.1 x 1521.2

Reprocessing Data

About Reprocessing

Version:

version unreported

Reprocessing failed.

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.9184

Sweep:

SWEEP2

Files:

/programs/datagrid/899/173A2_2.####.img

Images:

1 - 380

Beam:

(154.78, 160.27) -> (154.78, 161.5)

Distance:

130.0 -> 129.75

Spacegroup:

P 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.08 2.93 1.08
Low resolution limit 44.63 44.67 1.1
Completeness 93.5 94.2 89.3
Multiplicity 3.6 3.3 2.1
I/sigma 0.9 1.5 0.1
Rmerge 0.572 0.561 4.47
CC half 0.519 0.436 0.061
Anomalous completeness 86.2 88.1 59.8
Anomalous multiplicity 1.9 1.9 1.9
Cell 44.41 51.53 92.32 91.72 103.13 110.45

Version:

version unreported

Reprocessing failed.

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.9184

Sweep:

SWEEP2

Files:

/programs/datagrid/899/173A2_2.####.img

Images:

1 - 380

Beam:

(154.78, 160.27) -> (154.78, 161.49)

Distance:

130.0 -> 129.69

Spacegroup:

P 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.08 2.93 1.08
Low resolution limit 89.32 89.63 1.1
Completeness 94.4 98.8 89.4
Multiplicity 4.1 4.2 4.1
I/sigma 5.8 52.7 0.1
Rmerge 0.068 0.028 2.142
CC half 0.997 0.995 0.462
Anomalous completeness 92.6 97.7 86.8
Anomalous multiplicity 2.1 2.1 2.1
Cell 44.307 51.503 92.336 91.863 103.014 110.223

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.