X-Ray Diffraction data from Human Tubulin Acetyltransferase in complex with bisubstrate analog, source of 4PK2 structure

Data DOI: 10.15785/SBGRID/86 | ID: 86

Publication DOI: 10.1016/j.cell.2014.03.061

4PK2 Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Roll-Mecak Laboratory, National Institutes of Health

Release Date: May 19, 2015

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/86

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/86 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/86 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/86 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/86 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 86 ; shasum -c files.sha'

Storage requirements: 4.4G

Biological Sample:

Human Tubulin Acetyltransferase in complex with bisubstrate analog

Dataset Type:

X-Ray Diffraction

Subject Composition:

Ligand, Protein

Collection Facility:

Advanced Light Source (ALS), beamline 5.0.1.

Data Creation Date:

Nov. 12, 2012

Related Datasets:

None


Cite this Dataset

Szyk, A; Roll-Mecak, A. 2015. "X-Ray Diffraction data for: Human Tubulin Acetyltransferase in complex with bisubstrate analog. PDB Code 4PK2", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/86.

Download Citation

Dataset Description

Native data set

Project Members

Name Additional Roles Affiliation While Working on the Project
Agnieszka SzykData Collector, DepositorNIH/NINDS
Antonina Roll-MecakPINational Institutes of Health

Reprocessing Instructions

The data set was indexed in P21 space group, 250 frames were processed with HKL2000 at 1.35A resolution

Reprocessing Data

About Reprocessing

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 1.0

Sweep:

SWEEP1

Files:

/programs/datagrid/86/arm5_9_8_###.img

Images:

1 - 250

Beam:

(154.49, 161.6) -> (154.49, 161.55)

Distance:

180.0 -> 180.3

Spacegroup:

P 1 21 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.22 3.31 1.22
Low resolution limit 35.42 35.44 1.24
Completeness 81.7 98.6 29.8
Multiplicity 4.5 4.8 2.5
I/sigma 15.8 37.2 0.9
Rmerge 0.052 0.034 0.722
CC half 0.998 0.998 0.455
Anomalous completeness 78.1 97.5 21.8
Anomalous multiplicity 2.4 2.4 2.4
Cell 37.024 52.082 50.042 90.0 106.91 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 1.0

Sweep:

SWEEP1

Files:

/programs/datagrid/86/arm5_9_8_###.img

Images:

1 - 250

Beam:

(154.49, 161.6) -> (154.49, 161.59)

Distance:

180.0 -> 180.06

Spacegroup:

P 1 21 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.19 3.23 1.19
Low resolution limit 23.92 23.93 1.21
Completeness 78.8 99.1 20.7
Multiplicity 4.7 4.9 2.1
I/sigma 16.9 37.9 0.8
Rmerge 0.036 0.024 0.974
CC half 0.999 0.999 0.349
Anomalous completeness 74.9 97.0 12.4
Anomalous multiplicity 2.4 2.4 2.4
Cell 37.02 52.02 50.0 90.0 106.9 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 1.0

Sweep:

SWEEP1

Files:

/programs/datagrid/86/arm5_9_8_###.img

Images:

1 - 250

Beam:

(154.49, 161.6) -> (154.49, 161.58)

Distance:

180.0 -> 180.04

Spacegroup:

P 1 21 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.19 3.23 1.19
Low resolution limit 35.42 35.44 1.21
Completeness 78.8 99.0 20.7
Multiplicity 4.7 4.9 2.1
I/sigma 16.9 37.9 0.8
Rmerge 0.036 0.024 0.964
CC half 0.999 0.999 0.33
Anomalous completeness 74.9 97.0 12.4
Anomalous multiplicity 2.4 2.4 2.4
Cell 37.02 52.02 50.0 90.0 106.9 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 1.0

Sweep:

SWEEP1

Files:

/programs/datagrid/86/arm5_9_8_###.img

Images:

1 - 250

Beam:

(154.49, 161.6) -> (154.49, 161.59)

Distance:

180.0 -> 179.84

Spacegroup:

P 1 21 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.41 6.54 2.41
Low resolution limit 35.38 35.38 2.45
Completeness 69.8 96.0 0.5
Multiplicity 4.7 4.9 1.0
I/sigma 33.8 39.1 13.1
Rmerge 0.027 0.024 0.0
CC half 0.999 0.999 0.0
Anomalous completeness 65.4 95.5 0.0
Anomalous multiplicity 2.5 2.5 2.5
Cell 36.969 51.975 49.962 90.0 106.867 90.0

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.