X-Ray Diffraction data from nanobody complex with IL2Rb, source of 7S2S structure

Data DOI: 10.15785/SBGRID/850 | ID: 850

Publication DOI: 10.1016/j.cell.2022.02.025

7S2S Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Garcia Laboratory, Stanford University

Release Date: March 25, 2022

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/850

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/850 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/850 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/850 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/850 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 850 ; shasum -c files.sha'

Storage requirements: 11G

Biological Sample:

nanobody complex with IL2Rb

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

SSRL beamline 12-2

Data Creation Date:

June 6, 2021

Related Datasets:

None


Cite this Dataset

Jude, KM; Garcia, KC; Glassman, CR; Garcia, K. 2022. "X-Ray Diffraction data for: nanobody complex with IL2Rb. PDB Code 7S2S", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/850.

Download Citation

Dataset Description

native dataset

Project Members

Name Additional Roles Affiliation While Working on the Project
Kevin M JudeData Collector, DepositorHHMI/Stanford University
K C GarciaHHMI/Stanford University
Caleb R GlassmanStanford University
K. Christopher GarciaPIStanford University

Reprocessing Instructions

processed with xdsme

Reprocessing Data

About Reprocessing

Version:

version unreported

Reprocessing failed.

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97946

Sweep:

SWEEP1

Files:

/programs/datagrid/850/D13_4_#####.cbf

Images:

1 - 1800

Beam:

(217.32, 211.82) -> (217.32, 211.86)

Distance:

350.0 -> 340.69

Spacegroup:

P 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.79 4.86 1.79
Low resolution limit 40.86 40.87 1.82
Completeness 95.9 96.6 93.9
Multiplicity 3.6 3.5 3.7
I/sigma 7.1 26.2 0.6
Rmerge 0.093 0.031 1.804
CC half 0.996 0.998 0.342
Anomalous completeness 90.8 87.0 90.4
Anomalous multiplicity 1.8 1.8 1.8
Cell 42.69 69.5 91.71 99.57 99.86 90.19

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97946

Sweep:

SWEEP1

Files:

/programs/datagrid/850/D13_4_#####.cbf

Images:

1 - 1800

Beam:

(217.32, 211.82) -> (217.32, 211.95)

Distance:

350.0 -> 349.32

Spacegroup:

P 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.85 5.02 1.85
Low resolution limit 59.36 59.39 1.88
Completeness 95.9 96.5 94.9
Multiplicity 3.5 3.5 3.7
I/sigma 4.6 15.8 0.5
Rmerge 0.129 0.037 2.202
CC half 0.995 0.994 0.345
Anomalous completeness 90.6 85.8 91.3
Anomalous multiplicity 1.8 1.8 1.8
Cell 42.69 69.49 181.03 80.27 86.42 89.81

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97946

Sweep:

SWEEP1

Files:

/programs/datagrid/850/D13_4_#####.cbf

Images:

1 - 1800

Beam:

(217.32, 211.82) -> (217.32, 211.95)

Distance:

350.0 -> 349.63

Spacegroup:

P 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.73 4.69 1.73
Low resolution limit 50.32 50.34 1.76
Completeness 89.9 94.2 63.8
Multiplicity 3.6 3.6 3.5
I/sigma 6.1 34.0 0.2
Rmerge 0.089 0.036 1.846
CC half 0.997 0.996 0.294
Anomalous completeness 88.0 93.0 62.1
Anomalous multiplicity 1.8 1.8 1.8
Cell 42.678 69.468 91.68 99.599 99.843 90.191

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.