X-Ray Diffraction data from Human Coproporphyrinogen Oxidase, source of 2AEX structure

Data DOI: 10.15785/SBGRID/85 | ID: 85

Publication DOI: 10.1073/pnas.0506557102

2AEX Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Raman Laboratory, University of Maryland

Release Date: May 13, 2015

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/85

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/85 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/85 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/85 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/85 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 85 ; shasum -c files.sha'

Storage requirements: 1.6G

Biological Sample:

Human Coproporphyrinogen Oxidase

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

BL 9-2 Stanford Synchrotron Radiation Laboratories

Data Creation Date:

July 3, 2002

Related Datasets:

None


Cite this Dataset

Lee, D; Raman, CS. 2015. "X-Ray Diffraction data for: Human Coproporphyrinogen Oxidase. PDB Code 2AEX", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/85.

Download Citation

Dataset Description

This is the native data set obtained in two passes and requires merging to generate the complete dataset: hn_18: high resolution data (low res region masked with an aluminum disk) extending to 1.58 A or better hn_19: low resolution data (unattenuated) and can be readily merged to 1.8 A.

Project Members

Name Additional Roles Affiliation While Working on the Project
C S RamanData Collector, DepositorUniversity of Texas Medical School, Houston
Dong-Sun LeeData CollectorUniversity of Texas Medical School, Houston
C S RamanPIUniversity of Maryland

Reprocessing Instructions

none

Reprocessing Data

About Reprocessing

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.95369

Sweep:

SWEEP2

Files:

/programs/datagrid/85/hnlo_19_###.img

Images:

1 - 45

Beam:

(157.5, 157.5) -> (157.5, 157.51)

Distance:

180.0 -> 179.83

Spacegroup:

P 2 3

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.46 3.96 1.46
Low resolution limit 50.41 50.44 1.49
Completeness 99.6 99.9 99.0
Multiplicity 6.6 5.3 4.2
I/sigma 9.2 18.7 1.1
Rmerge 0.147 0.068 1.131
CC half 0.967 0.94 0.452
Anomalous completeness 94.1 92.9 93.1
Anomalous multiplicity 3.5 3.5 3.5
Cell 112.719 112.719 112.719 90.0 90.0 90.0

Version:

version unreported

Reprocessing failed.

Version:

version unreported

Reprocessing failed.

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.95369

Sweep:

SWEEP2

Files:

/programs/datagrid/85/hnlo_19_###.img

Images:

1 - 45

Beam:

(157.5, 157.5) -> (157.5, 156.23)

Distance:

180.0 -> 180.13

Spacegroup:

P 2 2 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.46 3.96 1.46
Low resolution limit 50.46 50.49 1.49
Completeness 87.1 93.4 76.3
Multiplicity 2.9 3.5 2.2
I/sigma 2.0 4.1 0.6
Rmerge 0.517 0.31 0.895
CC half 0.638 0.686 0.293
Anomalous completeness 67.6 80.8 51.9
Anomalous multiplicity 1.7 1.7 1.7
Cell 112.827 112.72 112.863 90.0 90.0 90.0

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.