X-Ray Diffraction data from Protein tyrosine phosphatase 1B, source of 7RIN structure

Data DOI: 10.15785/SBGRID/845 | ID: 845

7RIN Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Hekstra Laboratory, Harvard University

Release Date: Aug. 31, 2021

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/845

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/845 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/845 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/845 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/845 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 845 ; shasum -c files.sha'

Storage requirements: 17G

Biological Sample:

Protein tyrosine phosphatase 1B

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

Beamline 24-ID-C, NE-CAT, APS, Argonne National Laboratory

Data Creation Date:

July 17, 2019

Related Datasets:

None


Cite this Dataset

Greisman, JB; Hekstra, D. 2021. "X-Ray Diffraction data for: Protein tyrosine phosphatase 1B. PDB Code 7RIN", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/845.

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Dataset Description

Apo PTP1B by Native S-SAD at Room Temperature

Project Members

Name Additional Roles Affiliation While Working on the Project
Jack B GreismanData Collector, DepositorHarvard University
Doeke HekstraPIHarvard University

Reprocessing Instructions

This data was collected with the Pilatus detector raised by two panels. This altered the beam center from that reported in the image headers and introduced a shadow on the detector. For data reduction, the beam center should be set to (290.5, 225.2), and the pixel mask provided in the "Processing Bundle" should be used. The experiment was conducted at 6.5 keV to improve the anomalous signal, but this introduced an additional harmonic in the incident X-ray beam. The resulting secondary diffraction pattern is only observed at low resolution, and was excluded from DIALS spot finding, indexing, and geometry refinement by setting d_max to 6 angstroms. The data was collected from two crystals, using one 1440 pass on each. Both passes were processed separately with DIALS and scaled/merged together with AIMLESS to yield the final merged intensities.

Processing Bundle


License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.