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X-Ray Diffraction data from Putative DNA repair helicase RadD, with ADP, at 2.03 Angstrom., source of 7R7J structure

Data DOI: 10.15785/SBGRID/844 | ID: 844

7R7J Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Keck Laboratory, University of Wisconsin-Madison

Release Date: April 1, 2022

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/844

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/844 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/844 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/844 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/844 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 844 ; shasum -c files.sha'

Storage requirements: 12G

Biological Sample:

Putative DNA repair helicase RadD, with ADP, at 2.03 Angstrom.

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

21-ID-D

Data Creation Date:

June 4, 2019

Related Datasets:

None


Cite this Dataset

Satyshur, KA; Osorio Garcia, MA; Keck, JL. 2022. "X-Ray Diffraction data for: Putative DNA repair helicase RadD, with ADP, at 2.03 Angstrom.. PDB Code 7R7J", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/844.

Download Citation

Dataset Description

Collected at APS on beamline LS-Cat D. 0-360 degrees rotation one degree frames. MARR 300 CCD.

Project Members

Name Additional Roles Affiliation While Working on the Project
Kenneth A SatyshurDepositorSenior Scientist
Miguel A Osorio GarciaData CollectorGraduate Student
James L KeckPIUniversity of Wisconsin-Madison

Reprocessing Instructions

Processed and scaled with HKL2000. Indexing suggested no symmetry, thus P1.

Processing Bundle

Reprocessing Data

About Reprocessing

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97856

Sweep:

SWEEP1

Files:

/programs/datagrid/844/RadD_2_2_6.###

Images:

1 - 360

Beam:

(146.7, 155.25) -> (146.7, 155.04)

Distance:

300.0 -> 292.27

Spacegroup:

P 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.08 5.64 2.08
Low resolution limit 53.56 53.58 2.12
Completeness 96.7 94.0 94.8
Multiplicity 3.9 3.7 3.5
I/sigma 9.0 26.0 0.9
Rmerge 0.085 0.033 1.419
CC half 0.996 0.995 0.289
Anomalous completeness 91.4 81.0 92.1
Anomalous multiplicity 2.0 2.0 2.0
Cell 44.36 74.1 109.05 84.61 80.07 82.71

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97856

Sweep:

SWEEP1

Files:

/programs/datagrid/844/RadD_2_2_6.###

Images:

1 - 360

Beam:

(146.7, 155.25) -> (146.7, 155.03)

Distance:

300.0 -> 291.64

Spacegroup:

P 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.08 5.64 2.08
Low resolution limit 58.52 58.54 2.12
Completeness 96.7 94.0 94.8
Multiplicity 3.9 3.7 3.5
I/sigma 9.0 26.0 0.9
Rmerge 0.085 0.033 1.428
CC half 0.996 0.995 0.295
Anomalous completeness 91.4 81.1 92.2
Anomalous multiplicity 2.0 2.0 2.0
Cell 44.36 74.1 109.05 84.61 80.06 82.71

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97856

Sweep:

SWEEP1

Files:

/programs/datagrid/844/RadD_2_2_6.###

Images:

1 - 360

Beam:

(146.7, 155.25) -> (146.7, 155.11)

Distance:

300.0 -> 289.83

Spacegroup:

P 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.06 5.59 2.06
Low resolution limit 106.96 107.1 2.1
Completeness 98.1 99.7 95.2
Multiplicity 4.0 4.0 3.4
I/sigma 10.4 65.7 1.5
Rmerge 0.168 0.086 1.072
CC half 0.914 0.92 0.196
Anomalous completeness 97.8 99.7 92.0
Anomalous multiplicity 2.0 2.0 2.0
Cell 44.316 74.005 108.883 84.607 80.071 82.764

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.