X-Ray Diffraction data from SARS-CoV-2 N protein RNA-binding domain bound to single-domain antibody B6, source of 7R98 structure

Data DOI: 10.15785/SBGRID/837 | ID: 837

Publication DOI: 10.3389/fimmu.2021.719037

7R98 Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Corbett Laboratory, University of California, San Diego

Release Date: Feb. 8, 2022

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/837

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/837 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/837 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/837 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/837 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 837 ; shasum -c files.sha'

Storage requirements: 31G

Biological Sample:

SARS-CoV-2 N protein RNA-binding domain bound to single-domain antibody B6

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

APS 24ID-C

Data Creation Date:

March 24, 2021

Related Datasets:

None


Cite this Dataset

Ye, Q; Corbett, KD. 2022. "X-Ray Diffraction data for: SARS-CoV-2 N protein RNA-binding domain bound to single-domain antibody B6. PDB Code 7R98", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/837.

Download Citation

Dataset Description

Native dataset

Project Members

Name Additional Roles Affiliation While Working on the Project
Kevin D CorbettDepositorUC San Diego
Qiaozhen YeData CollectorUC San Diego
Kevin CorbettPIUniversity of California, San Diego

Reprocessing Instructions

none

Reprocessing Data

About Reprocessing

Version:

version unreported

Reprocessing failed.

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97918

Sweep:

SWEEP1

Files:

/programs/datagrid/837/9_16_1_######.cbf

Images:

1 - 1800

Beam:

(164.62, 155.36) -> (164.62, 155.31)

Distance:

300.0 -> 301.31

Spacegroup:

P 21 21 21

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.6 7.05 2.6
Low resolution limit 48.82 48.83 2.64
Completeness 100.0 100.0 100.0
Multiplicity 13.8 13.0 14.4
I/sigma 4.4 10.8 0.4
Rmerge 0.32 0.105 6.426
CC half 0.996 0.998 0.339
Anomalous completeness 99.8 99.9 99.9
Anomalous multiplicity 7.2 7.2 7.2
Cell 99.65 112.0 175.11 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97918

Sweep:

SWEEP1

Files:

/programs/datagrid/837/9_16_1_######.cbf

Images:

1 - 1800

Beam:

(164.62, 155.36) -> (164.62, 155.33)

Distance:

300.0 -> 299.72

Spacegroup:

P 1 21 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.51 6.81 2.51
Low resolution limit 86.58 86.63 2.55
Completeness 99.9 99.9 99.8
Multiplicity 7.1 6.8 6.6
I/sigma 6.8 18.3 0.6
Rmerge 0.175 0.077 2.529
CC half 0.994 0.995 0.299
Anomalous completeness 99.5 99.2 99.3
Anomalous multiplicity 3.6 3.6 3.6
Cell 99.48 111.86 100.46 90.0 119.5 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97918

Sweep:

SWEEP1

Files:

/programs/datagrid/837/9_16_1_######.cbf

Images:

1 - 1800

Beam:

(164.62, 155.36) -> (164.62, 155.33)

Distance:

300.0 -> 300.67

Spacegroup:

C 2 2 21

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.34 6.35 2.34
Low resolution limit 112.04 112.16 2.38
Completeness 100.0 100.0 100.0
Multiplicity 13.2 12.3 13.1
I/sigma 4.6 21.9 0.1
Rmerge 0.241 0.107 5.019
CC half 0.994 0.994 0.317
Anomalous completeness 100.0 100.0 100.0
Anomalous multiplicity 6.9 6.9 6.9
Cell 99.715 175.22 112.084 90.0 90.0 90.0

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.