X-Ray Diffraction data from SARS-CoV-2 N protein C-terminal domain bound to single-domain antibody E2, source of 7N0I structure

Data DOI: 10.15785/SBGRID/835 | ID: 835

Publication DOI: 10.3389/fimmu.2021.719037

7N0I Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Corbett Laboratory, University of California, San Diego

Release Date: Feb. 8, 2022

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/835

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/835 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/835 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/835 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/835 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 835 ; shasum -c files.sha'

Storage requirements: 4.2G

Biological Sample:

SARS-CoV-2 N protein C-terminal domain bound to single-domain antibody E2

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

Advanced Light Source BL 5.0.2

Data Creation Date:

May 14, 2021

Related Datasets:

None


Cite this Dataset

Ye, Q; Corbett, KD. 2022. "X-Ray Diffraction data for: SARS-CoV-2 N protein C-terminal domain bound to single-domain antibody E2. PDB Code 7N0I", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/835.

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Dataset Description

Native dataset

Project Members

Name Additional Roles Affiliation While Working on the Project
Kevin D CorbettDepositorUC San Diego
Qiaozhen YeData CollectorUC San Diego
Kevin CorbettPIUniversity of California, San Diego

Reprocessing Instructions

Process to 1.8 A; refine to 2.2 A

Reprocessing Data

About Reprocessing

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 1.00004

Sweep:

SWEEP1

Files:

/programs/datagrid/835/8_2_1_#####.cbf

Images:

1 - 720

Beam:

(220.85, 213.37) -> (220.85, 213.02)

Distance:

300.0 -> 304.74

Spacegroup:

P 21 21 21

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.81 4.91 1.81
Low resolution limit 46.94 46.95 1.84
Completeness 99.9 100.0 99.9
Multiplicity 6.6 6.3 6.4
I/sigma 8.3 29.7 0.5
Rmerge 0.079 0.038 4.336
CC half 0.999 0.998 0.365
Anomalous completeness 98.5 98.5 99.1
Anomalous multiplicity 3.4 3.4 3.4
Cell 76.02 131.85 140.38 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 1.00004

Sweep:

SWEEP1

Files:

/programs/datagrid/835/8_2_1_#####.cbf

Images:

1 - 720

Beam:

(220.85, 213.37) -> (220.85, 213.04)

Distance:

300.0 -> 305.2

Spacegroup:

P 21 21 21

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.81 4.91 1.81
Low resolution limit 48.05 48.07 1.84
Completeness 99.9 100.0 99.9
Multiplicity 6.6 6.3 6.4
I/sigma 8.3 29.6 0.5
Rmerge 0.079 0.038 4.347
CC half 0.999 0.999 0.358
Anomalous completeness 98.5 98.4 99.1
Anomalous multiplicity 3.4 3.4 3.4
Cell 76.02 131.85 140.38 90.0 90.0 90.0

Version:

version unreported

Reprocessing failed.


License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.