Micro-Electron Diffraction data from Human carbonic anhydrase II complexed with acetazolamide, source of 6YMA structure

Data DOI: 10.15785/SBGRID/792 | ID: 792

Publication DOI: 10.1038/s42003-020-01155-1

6YMA Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Zou Laboratory, Stockholm University

Release Date: Aug. 4, 2020

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/792

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/792 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/792 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/792 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/792 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 792 ; shasum -c files.sha'

Storage requirements: 166M

Biological Sample:

Human carbonic anhydrase II complexed with acetazolamide

Dataset Type:

Micro-Electron Diffraction

Subject Composition:

Ligand, Protein

Collection Facility:

JEOL JEM 2100 (LaB6)

Data Creation Date:

Oct. 23, 2019

Related Datasets:

None


Cite this Dataset

Clabbers, MT.B.; Xu, H; Zou, X. 2020. "Micro-Electron Diffraction data for: Human carbonic anhydrase II complexed with acetazolamide. PDB Code 6YMA", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/792.

Download Citation

Dataset Description

Microcrystal electron diffraction data of HCA II:AZM

Project Members

Name Additional Roles Affiliation While Working on the Project
Max T.B. ClabbersData Collector, DepositorStockholm University
Hongyi XuData CollectorStockholm University
Xiaodong ZouPI Stockholm University

Reprocessing Instructions

Microcrystal electron diffraction (MicroED) can be processed using standard crystallographic software such as XDS[1], DIALS[2,3], and MOSFLM[4]. The HCA II:AZM diffraction data were processed and merged using XDS. The detector geometry is defined as following: NX=516 NY=516 QX=0.055 QY=0.055 Pixels 256-261 in both x- and y-direction were masked using: UNTRUSTED_RECTANGLE=255 262 0 517 UNTRUSTED_RECTANGLE=0 517 255 262 The essential experimental parameters are: DETECTOR_DISTANCE=1481.74 ROTATION_AXIS=-0.6204 0.7843 0.0000 OSCILLATION_RANGE=0.68 [1] Kabsch, _Acta Cryst_ D66, 125–32 (2010) [2] Winter _et al._, _Acta Cryst._ D74, 85–97 (2018) [3] Clabbers _et al._, _Acta Cryst._ D74, 506–518 (2018) [4] Leslie, _Acta Cryst._ D62, 48–57 (2006)


License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.