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X-Ray Diffraction data from Human CRM1 (C-terminal truncation mutant), source of 4BSN structure

Data DOI: 10.15785/SBGRID/77 | ID: 77

Publication DOI: 10.1016/j.str.2013.06.003

4BSN Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Petosa Laboratory, Institut de Biologie Structurale, Grenoble

Release Date: May 11, 2015

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/77

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/77 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/77 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/77 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/77 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 77 ; shasum -c files.sha'

Storage requirements: 16G

Biological Sample:

Human CRM1 (C-terminal truncation mutant)

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

ESRF beamline ID23-2

Data Creation Date:

April 25, 2009

Related Datasets:

None


Cite this Dataset

Langer, K; Petosa, C. 2015. "X-Ray Diffraction data for: Human CRM1 (C-terminal truncation mutant). PDB Code 4BSN", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/77.

Download Citation

Dataset Description

Native data for crystal variant 1: low resolution and high-resolution passes

Project Members

Name Additional Roles Affiliation While Working on the Project
Carlo PetosaData Collector, DepositorInstitut de Biologie Structurale, Grenoble, France
Karla LangerData CollectorInstitut de Biologie Structurale, Grenoble, France
Carlo PetosaPIInstitut de Biologie Structurale, Grenoble

Reprocessing Instructions

Crystals were highly sensitive to radiation damage and diffraction quality decayed rapidly. First, a low-resolution pass was collected in a single sweep of 180 degrees. (Files are named "lowres_1_xxx.mccd") Subsequently, several different volumes from the same crystal were exposed to higher dose over smaller oscillation ranges. (Files are named "highres_?_xxx.mccd", where ? is the run number.) Runs 8-11 were collected from a single crystal volume, whereas the other runs (1,2,4,5,6,7; there is no run 3) are from different volumes (i.e. a total of 7 crystal volumes were shot). The oscillation range for each image is given in the log file corresponding to each run. The structure factors deposited in the PDB were obtained from these image data by: i) integrating data from the different runs using different resolution limits (i.e., using lower resolution for those volumes that diffracted more poorly) and ii) integrating earlier images within each run using higher resolution limits than later images.


License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.