Data DOI: 10.15785/SBGRID/77 | ID: 77
Publication DOI: 10.1016/j.str.2013.06.003
4BSN Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB
Petosa Laboratory, Institut de Biologie Structurale, Grenoble
Release Date: May 11, 2015
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'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/77 .' (Harvard Medical School, USA)
Depending on your location, faster access may be available from a Tier 1 site closer to your location
'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/77 .' (Uppsala University, Sweden)
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3. After the transfer is completed, please issue the following command to verify data integrity:
'cd 77 ; shasum -c files.sha'
Storage requirements: 16G
Human CRM1 (C-terminal truncation mutant)
ESRF beamline ID23-2
Data Creation Date:
April 25, 2009
Langer, K; Petosa, C. 2015. "X-Ray Diffraction data for: Human CRM1 (C-terminal truncation mutant). PDB Code 4BSN", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/77.
Native data for crystal variant 1: low resolution and high-resolution passes
|Name||Additional Roles||Affiliation While Working on the Project|
|Carlo Petosa||Data Collector, Depositor||Institut de Biologie Structurale, Grenoble, France|
|Karla Langer||Data Collector||Institut de Biologie Structurale, Grenoble, France|
|Carlo Petosa||PI||Institut de Biologie Structurale, Grenoble|
Crystals were highly sensitive to radiation damage and diffraction quality decayed rapidly. First, a low-resolution pass was collected in a single sweep of 180 degrees. (Files are named "lowres_1_xxx.mccd") Subsequently, several different volumes from the same crystal were exposed to higher dose over smaller oscillation ranges. (Files are named "highres_?_xxx.mccd", where ? is the run number.) Runs 8-11 were collected from a single crystal volume, whereas the other runs (1,2,4,5,6,7; there is no run 3) are from different volumes (i.e. a total of 7 crystal volumes were shot). The oscillation range for each image is given in the log file corresponding to each run. The structure factors deposited in the PDB were obtained from these image data by: i) integrating data from the different runs using different resolution limits (i.e., using lower resolution for those volumes that diffracted more poorly) and ii) integrating earlier images within each run using higher resolution limits than later images.
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