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The SBDB will be operating at a reduced level due to underlying network maintenance, starting July 24. New dataset registrations, deposition credential requests, dataset uploads, and dataset downloads from HMS will be unavailable during this time. Local access and downloads from other DAA sites will be unaffected. We expect to return to full operations July 25 or earlier.

X-Ray Diffraction data from NKp30 in complex with B7H6, source of 6YJP structure

Data DOI: 10.15785/SBGRID/753 | ID: 753

Publication DOI: 10.3390/cancers12071998

6YJP Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Dohnalek Laboratory, Institute of Biotechnology of the Czech Academy of Sciences

Release Date: July 17, 2020

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/753

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/753 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/753 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/753 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/753 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 753 ; shasum -c files.sha'

Storage requirements: 95G

Biological Sample:

NKp30 in complex with B7H6

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

Diamond, beamline I02

Data Creation Date:

June 29, 2016

Related Datasets:

None


Cite this Dataset

Skalova, T; Harlos, K; Skorepa, O; Vanek, O; Dohnalek, J. 2020. "X-Ray Diffraction data for: NKp30 in complex with B7H6. PDB Code 6YJP", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/753.

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Dataset Description

six data sets (six crystals) used for merging and molecular replacement (no experimental phasing)

Project Members

Name Additional Roles Affiliation While Working on the Project
Tereza SkalovaData Collector, DepositorInstitute of Biotechnology of the Czech Academy of Sciences, v.v.i., Prumyslova 595, 252 50, Vestec, Czech Republic
Jan DohnalekData CollectorInstitute of Biotechnology of the Czech Academy of Sciences, v.v.i., Prumyslova 595, 252 50, Vestec, Czech Republic
Karl HarlosData CollectorDivision of Structural Biology, The Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, OX3 7BN Oxford, United Kingdom
Ondrej SkorepaData CollectorDepartment of Biochemistry, Faculty of Science, Charles University Prague, Hlavova 8, 128 40 Praha, Czech Republic
Ondrej VanekData CollectorDepartment of Biochemistry, Faculty of Science, Charles University Prague, Hlavova 8, 128 40 Praha, Czech Republic
Jan DohnalekPIInstitute of Biotechnology of the Czech Academy of Sciences

Reprocessing Instructions

Merging strategies tested: 1) B7x1 (800 images), B8x5 (800 images) and B8x3 (450 images), 3.1A in C2; 2) B6x2 (800), B7x1 (1800), B8x2 (2400), B8x3 (2200), B8x4 (2100), B8x5 (1800), 3.4A in P1; Finally, three data sets (B7x1: images 1 to 800, B8x5:1 to 800, and B8x3:1 to 450) were merged with the best results (space group C2, 3.1A).


License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.