X-Ray Diffraction data from Exomer (Chs5/Bch1) / Arf1 complex, source of 4Q66 structure

Data DOI: 10.15785/SBGRID/75 | ID: 75

Publication DOI: 10.1016/j.devcel.2014.07.014

4Q66 Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Fromme Laboratory, Cornell University

Release Date: May 11, 2015

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/75

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/75 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/75 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/75 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/75 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 75 ; shasum -c files.sha'

Storage requirements: 8.2G

Biological Sample:

Exomer (Chs5/Bch1) / Arf1 complex

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

CHESS A1

Data Creation Date:

July 2, 2012

Related Datasets:

None


Cite this Dataset

Paczkowski, JE.; Fromme, J. 2015. "X-Ray Diffraction data for: Exomer (Chs5/Bch1) / Arf1 complex. PDB Code 4Q66", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/75.

Download Citation

Dataset Description

Native dataset.

Project Members

Name Additional Roles Affiliation While Working on the Project
Jon E. PaczkowskiData CollectorCornell University
J. Christopher FrommeData Collector, Depositor, PICornell University

Reprocessing Instructions

We provide the first 260 images of the dataset, which were used instead of the full 360 images collected, due to signs of radiation damage. HKL2000 was used to index, scale, and reduce the data.

Reprocessing Data

About Reprocessing

Version:

version unreported

Reprocessing failed.

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.976

Sweep:

SWEEP1

Files:

/programs/datagrid/75/crystal2_1_###.img

Images:

1 - 260

Beam:

(109.34, 109.39) -> (109.34, 109.94)

Distance:

300.0 -> 296.45

Spacegroup:

P 1 21 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 4.37 11.76 4.37
Low resolution limit 39.85 39.86 4.45
Completeness 97.3 92.2 99.0
Multiplicity 2.7 2.6 2.8
I/sigma 9.7 17.5 5.7
Rmerge 0.051 0.029 0.142
CC half 0.991 0.977 0.975
Anomalous completeness 89.5 81.1 92.9
Anomalous multiplicity 1.5 1.5 1.5
Cell 166.41 208.09 215.82 90.0 107.71 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.976

Sweep:

SWEEP1

Files:

/programs/datagrid/75/crystal2_1_###.img

Images:

1 - 260

Beam:

(109.34, 109.39) -> (109.34, 109.93)

Distance:

300.0 -> 296.06

Spacegroup:

P 1 21 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 4.42 11.89 4.42
Low resolution limit 39.65 39.65 4.5
Completeness 97.7 94.0 99.4
Multiplicity 2.8 2.7 2.8
I/sigma 17.0 27.4 10.7
Rmerge 0.036 0.027 0.076
CC half 0.991 0.97 0.98
Anomalous completeness 90.5 87.0 92.9
Anomalous multiplicity 1.5 1.5 1.5
Cell 114.53 208.17 155.09 90.0 105.43 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.976

Sweep:

SWEEP1

Files:

/programs/datagrid/75/crystal2_1_###.img

Images:

1 - 260

Beam:

(109.34, 109.39) -> (109.34, 116.34)

Distance:

300.0 -> 258.77

Spacegroup:

P 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 3.94 10.68 3.94
Low resolution limit 76.94 76.96 4.01
Completeness 77.4 72.2 76.2
Multiplicity 1.8 1.8 1.7
I/sigma 0.7 1.8 0.3
Rmerge -5.067 0.0 -0.94
CC half -0.008 -0.059 -0.075
Anomalous completeness 58.9 57.3 54.5
Anomalous multiplicity 1.0 1.0 1.0
Cell 101.482 138.417 188.356 90.0 90.0 105.415

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.