X-Ray Diffraction data from Crystal structures of the LiaR DNA binding domain with secondary DNA target sequence, source of 4WUL structure

Data DOI: 10.15785/SBGRID/72 | ID: 72

Publication DOI: 10.1093/nar/gkv321

4WUL Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Shamoo Laboratory, Rice University

Release Date: May 10, 2015

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/72

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/72 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/72 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/72 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/72 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 72 ; shasum -c files.sha'

Storage requirements: 12G

Biological Sample:

Crystal structures of the LiaR DNA binding domain with secondary DNA target sequence

Dataset Type:

X-Ray Diffraction

Subject Composition:

DNA, Protein

Collection Facility:

APS BEAMLINE 21-ID-D

Data Creation Date:

April 19, 2014

Related Datasets:

None


Cite this Dataset

Davlieva, M; Shamoo, Y; Shamoo, Y. 2015. "X-Ray Diffraction data for: Crystal structures of the LiaR DNA binding domain with secondary DNA target sequence. PDB Code 4WUL", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/72.

Download Citation

Dataset Description

Native data sets

Project Members

Name Additional Roles Affiliation While Working on the Project
Milya DavlievaData Collector, DepositorDepartment of BioSciences, Rice University, Houston, TX 77005, USA
Yousif ShamooData CollectorDepartment of BioSciences, Rice University, Houston, TX 77005, USA
Yousif ShamooPIRice University

Reprocessing Instructions

This data set was indexed in space group P62 and was processed with HKL2000 at 2.4 A

Reprocessing Data

About Reprocessing

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97883

Sweep:

SWEEP1

Files:

/programs/datagrid/72/data2.###

Images:

1 - 360

Beam:

(150.0, 150.0) -> (150.0, 150.03)

Distance:

300.0 -> 300.64

Spacegroup:

P 62

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.04 5.54 2.04
Low resolution limit 49.23 49.24 2.08
Completeness 100.0 100.0 100.0
Multiplicity 21.4 19.5 14.3
I/sigma 11.9 26.8 1.0
Rmerge 0.176 0.074 4.38
CC half 0.998 0.998 0.322
Anomalous completeness 100.0 100.0 100.0
Anomalous multiplicity 11.0 11.0 11.0
Cell 113.683 113.683 48.197 90.0 90.0 120.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97883

Sweep:

SWEEP1

Files:

/programs/datagrid/72/data2.###

Images:

1 - 360

Beam:

(150.0, 150.0) -> (150.0, 149.92)

Distance:

300.0 -> 300.49

Spacegroup:

P 62

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.12 5.75 2.12
Low resolution limit 56.86 56.88 2.16
Completeness 99.8 100.0 100.0
Multiplicity 22.2 19.5 21.7
I/sigma 21.4 56.4 1.6
Rmerge 0.096 0.042 2.937
CC half 0.999 0.999 0.532
Anomalous completeness 99.7 99.6 99.6
Anomalous multiplicity 11.5 11.5 11.5
Cell 113.72 113.72 48.22 90.0 90.0 120.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97883

Sweep:

SWEEP1

Files:

/programs/datagrid/72/data2.###

Images:

1 - 360

Beam:

(150.0, 150.0) -> (150.0, 149.92)

Distance:

300.0 -> 300.4

Spacegroup:

P 62

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.12 5.75 2.12
Low resolution limit 98.48 98.59 2.16
Completeness 99.8 100.0 100.0
Multiplicity 22.2 19.5 21.7
I/sigma 21.4 56.3 1.6
Rmerge 0.096 0.042 2.922
CC half 0.999 0.999 0.524
Anomalous completeness 99.8 99.6 100.0
Anomalous multiplicity 11.5 11.5 11.5
Cell 113.72 113.72 48.23 90.0 90.0 120.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97883

Sweep:

SWEEP1

Files:

/programs/datagrid/72/data2.###

Images:

1 - 360

Beam:

(150.0, 150.0) -> (150.0, 150.0)

Distance:

300.0 -> 300.6

Spacegroup:

P 62

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.19 5.94 2.19
Low resolution limit 98.58 98.68 2.23
Completeness 99.9 100.0 97.7
Multiplicity 22.6 20.5 22.7
I/sigma 12.7 25.8 1.9
Rmerge 0.147 0.06 18.364
CC half 0.998 0.999 0.832
Anomalous completeness 99.9 99.9 97.9
Anomalous multiplicity 11.7 11.7 11.7
Cell 113.826 113.826 48.288 90.0 90.0 120.0

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.