X-Ray Diffraction data from Complex of Notch1 (EGF11-13) bound to Delta-like 4 (N-EGF2) , source of 4XLW structure

Data DOI: 10.15785/SBGRID/69 | ID: 69

Publication DOI: 10.1126/science.1261093

4XLW Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Garcia Laboratory, Stanford University

Release Date: May 8, 2015

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/69

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/69 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/69 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/69 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/69 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 69 ; shasum -c files.sha'

Storage requirements: 3.2G

Biological Sample:

Complex of Notch1 (EGF11-13) bound to Delta-like 4 (N-EGF2)

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

ALS beamline 8.2.2

Data Creation Date:

April 12, 2014

Related Datasets:

None


Cite this Dataset

Jude, KM; Garcia, KC; Luca, VC; Garcia, K. 2015. "X-Ray Diffraction data for: Complex of Notch1 (EGF11-13) bound to Delta-like 4 (N-EGF2) . PDB Code 4XLW", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/69.

Download Citation

Dataset Description

Native dataset of Notch bound to Delta-like 4

Project Members

Name Additional Roles Affiliation While Working on the Project
Kevin M JudeDepositorStanford University
K C GarciaStanford University
Vincent C LucaData CollectorStanford University
K. Christopher GarciaPIStanford University

Reprocessing Instructions

processed with XDS to 3.4 Å resolution

Reprocessing Data

About Reprocessing

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.99996

Sweep:

SWEEP1

Files:

/programs/datagrid/69/2egf_1_###.img

Images:

1 - 180

Beam:

(155.1, 163.45) -> (155.1, 163.25)

Distance:

350.0 -> 351.23

Spacegroup:

P 1 21 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 4.37 11.85 4.37
Low resolution limit 84.15 84.17 4.45
Completeness 100.0 100.0 100.0
Multiplicity 3.5 3.5 3.5
I/sigma 7.4 15.7 3.7
Rmerge 0.096 0.042 0.275
CC half 0.998 0.998 0.946
Anomalous completeness 97.2 99.1 95.7
Anomalous multiplicity 1.8 1.8 1.8
Cell 84.638 95.661 123.407 90.0 96.147 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.99996

Sweep:

SWEEP1

Files:

/programs/datagrid/69/2egf_1_###.img

Images:

1 - 180

Beam:

(155.1, 163.45) -> (155.1, 163.22)

Distance:

350.0 -> 352.29

Spacegroup:

P 1 21 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 3.58 9.71 3.58
Low resolution limit 73.04 73.05 3.64
Completeness 99.2 94.9 99.5
Multiplicity 3.7 3.5 3.7
I/sigma 6.6 21.2 1.4
Rmerge 0.13 0.027 0.967
CC half 0.998 0.999 0.687
Anomalous completeness 94.3 87.3 95.1
Anomalous multiplicity 1.9 1.9 1.9
Cell 84.56 95.59 122.92 90.0 96.19 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.99996

Sweep:

SWEEP1

Files:

/programs/datagrid/69/2egf_1_###.img

Images:

1 - 180

Beam:

(155.1, 163.45) -> (155.1, 163.21)

Distance:

350.0 -> 350.64

Spacegroup:

P 1 21 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 3.64 9.88 3.64
Low resolution limit 122.24 122.31 3.7
Completeness 99.2 94.6 98.7
Multiplicity 3.7 3.5 3.7
I/sigma 6.9 21.5 1.7
Rmerge 0.123 0.027 0.732
CC half 0.998 0.999 0.682
Anomalous completeness 94.2 86.8 91.3
Anomalous multiplicity 1.9 1.9 1.9
Cell 84.58 95.61 122.96 90.0 96.19 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.99996

Sweep:

SWEEP1

Files:

/programs/datagrid/69/2egf_1_###.img

Images:

1 - 180

Beam:

(155.1, 163.45) -> (155.1, 163.22)

Distance:

350.0 -> 353.26

Spacegroup:

P 1 21 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 4.7 12.75 4.7
Low resolution limit 96.02 96.04 4.78
Completeness 99.6 100.0 91.8
Multiplicity 3.6 3.6 3.6
I/sigma 6.7 11.9 4.1
Rmerge 0.091 0.037 0.245
CC half 0.998 0.999 0.95
Anomalous completeness 98.2 100.0 88.3
Anomalous multiplicity 1.9 1.9 1.9
Cell 84.994 96.015 123.873 90.0 96.161 90.0

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.