X-Ray Diffraction data from Designed repeat protein specifically in complex with Fz7CRD, source of 6NE4 structure

Data DOI: 10.15785/SBGRID/660 | ID: 660

Publication DOI: 10.1038/s41594-019-0224-z

6NE4 Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Garcia Laboratory, Stanford University

Release Date: May 24, 2019

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/660

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/660 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/660 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/660 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/660 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 660 ; shasum -c files.sha'

Storage requirements: 5.3G

Biological Sample:

Designed repeat protein specifically in complex with Fz7CRD

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

SSRL beamline 12-2

Data Creation Date:

July 23, 2017

Related Datasets:

None


Cite this Dataset

Jude, KM; Garcia, KC; Miao, Y; Garcia, K. 2019. "X-Ray Diffraction data for: Designed repeat protein specifically in complex with Fz7CRD. PDB Code 6NE4", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/660.

Download Citation

Dataset Description

native dataset

Project Members

Name Additional Roles Affiliation While Working on the Project
Kevin M JudeData Collector, DepositorStanford University/HHMI
K C GarciaStanford University/HHMI
Yi MiaoStanford University
K. Christopher GarciaPIStanford University

Reprocessing Instructions

images were processed with XDS and reflections merged with aimless

Reprocessing Data

About Reprocessing

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97946

Sweep:

SWEEP1

Files:

/programs/datagrid/660/B2_1_#####.cbf

Images:

1 - 900

Beam:

(217.32, 211.82) -> (217.32, 211.83)

Distance:

500.0 -> 496.26

Spacegroup:

P 21 21 21

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.3 6.21 2.3
Low resolution limit 23.97 23.97 2.34
Completeness 100.0 100.0 98.8
Multiplicity 6.2 5.4 4.4
I/sigma 8.1 23.8 0.7
Rmerge 0.147 0.05 1.784
CC half 0.996 0.997 0.369
Anomalous completeness 98.7 98.8 94.0
Anomalous multiplicity 3.4 3.4 3.4
Cell 39.07 63.48 103.46 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97946

Sweep:

SWEEP1

Files:

/programs/datagrid/660/B2_1_#####.cbf

Images:

1 - 900

Beam:

(217.32, 211.82) -> (217.32, 211.86)

Distance:

500.0 -> 493.61

Spacegroup:

P 21 21 21

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.3 6.23 2.3
Low resolution limit 36.2 36.21 2.34
Completeness 100.0 100.0 99.3
Multiplicity 6.2 5.3 4.7
I/sigma 7.8 22.9 0.7
Rmerge 0.156 0.051 2.003
CC half 0.995 0.997 0.383
Anomalous completeness 98.9 98.8 96.4
Anomalous multiplicity 3.4 3.4 3.4
Cell 38.69 62.91 102.67 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97946

Sweep:

SWEEP1

Files:

/programs/datagrid/660/B2_1_#####.cbf

Images:

1 - 900

Beam:

(217.32, 211.82) -> (217.32, 211.86)

Distance:

500.0 -> 498.45

Spacegroup:

P 21 21 21

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.21 5.99 2.21
Low resolution limit 36.53 36.53 2.25
Completeness 99.6 100.0 94.9
Multiplicity 6.0 5.6 3.5
I/sigma 7.6 30.6 0.4
Rmerge 0.155 0.065 1.59
CC half 0.995 0.996 0.215
Anomalous completeness 97.9 99.8 85.0
Anomalous multiplicity 3.3 3.3 3.3
Cell 39.04 63.468 103.516 90.0 90.0 90.0

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.