X-Ray Diffraction data from Dimeric E2C Darpin complex with EpoR, source of 6MOH structure

Data DOI: 10.15785/SBGRID/622 | ID: 622

Publication DOI: 10.1126/science.aav7532

6MOH Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Garcia Laboratory, Stanford University

Release Date: May 24, 2019

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/622

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/622 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/622 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/622 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/622 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 622 ; shasum -c files.sha'

Storage requirements: 6.4G

Biological Sample:

Dimeric E2C Darpin complex with EpoR

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

beamline 8.2.2, ALS

Data Creation Date:

Aug. 25, 2017

Related Datasets:

None


Cite this Dataset

Jude, KM; Garcia, KC; Mohan, K; Garcia, K. 2019. "X-Ray Diffraction data for: Dimeric E2C Darpin complex with EpoR. PDB Code 6MOH", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/622.

Download Citation

Dataset Description

Native dataset

Project Members

Name Additional Roles Affiliation While Working on the Project
Kevin M JudeData Collector, DepositorStanford University/Howard Hughes Medical Institute
K C GarciaStanford University, Howard Hughes Medical Institute
Kritika MohanStanford University
K. Christopher GarciaPIStanford University

Reprocessing Instructions

Beam center in image header is incorret; used ORGX= 1591.46 ORGY= 1468.55. Images 1-100 from dataset 1421-7_6 and images 1-180 from dataset 1721-6_5 were processed with XDS and scaled together. Data were ellipsoidally truncated with STARAniso for refinement.

Reprocessing Data

About Reprocessing

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.99992

Sweep:

SWEEP2

Files:

/programs/datagrid/622/1421-7_6_#####.img

Images:

1 - 182

Beam:

(154.5, 163.2) -> (154.5, 162.73)

Distance:

390.0 -> 391.15

Spacegroup:

P 4 21 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 3.16 8.57 3.16
Low resolution limit 67.76 67.77 3.21
Completeness 99.3 97.3 99.9
Multiplicity 11.8 12.7 7.4
I/sigma 2.9 9.8 0.2
Rmerge 0.561 0.171 7.108
CC half 0.979 0.993 0.269
Anomalous completeness 99.9 99.9 99.8
Anomalous multiplicity 6.5 6.5 6.5
Cell 96.08 96.08 286.69 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.99992

Sweep:

SWEEP2

Files:

/programs/datagrid/622/1421-7_6_#####.img

Images:

1 - 182

Beam:

(154.5, 163.2) -> (154.5, 162.72)

Distance:

390.0 -> 389.68

Spacegroup:

P 4 21 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 3.15 8.55 3.15
Low resolution limit 91.13 91.17 3.2
Completeness 99.3 97.3 99.7
Multiplicity 11.9 12.6 7.5
I/sigma 2.9 9.9 0.2
Rmerge 0.565 0.17 7.401
CC half 0.978 0.992 0.31
Anomalous completeness 99.9 99.9 99.6
Anomalous multiplicity 6.5 6.5 6.5
Cell 96.11 96.11 286.78 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.99992

Sweep:

SWEEP2

Files:

/programs/datagrid/622/1421-7_6_#####.img

Images:

1 - 182

Beam:

(154.5, 163.2) -> (154.5, 162.73)

Distance:

390.0 -> 388.32

Spacegroup:

P 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 3.72 10.1 3.72
Low resolution limit 95.65 95.68 3.79
Completeness 77.9 74.1 69.4
Multiplicity 2.5 2.7 2.4
I/sigma 1.8 8.7 0.7
Rmerge 0.83 0.197 4.779
CC half 0.611 0.92 0.115
Anomalous completeness 64.2 68.7 54.6
Anomalous multiplicity 1.4 1.4 1.4
Cell 95.588 95.651 286.038 90.139 89.814 90.049

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.