X-Ray Diffraction data from Human GABA(B) receptor extracellular domain in complex with the antagonist 2-hydroxysaclofen, source of 4MQF structure

Data DOI: 10.15785/SBGRID/59 | ID: 59

Publication DOI: 10.1038/nature12725

4MQF Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Fan Laboratory, Columbia University

Release Date: May 7, 2015

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/59

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/59 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/59 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/59 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/59 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 59 ; shasum -c files.sha'

Storage requirements: 3.2G

Biological Sample:

Human GABA(B) receptor extracellular domain in complex with the antagonist 2-hydroxysaclofen

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

Beamline 24-ID-C, NE-CAT, APS, Chicago, IL

Data Creation Date:

Aug. 16, 2011

Related Datasets:

None


Cite this Dataset

Geng, Y; Mosyak, L; Fan, QR. 2015. "X-Ray Diffraction data for: Human GABA(B) receptor extracellular domain in complex with the antagonist 2-hydroxysaclofen. PDB Code 4MQF", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/59.

Download Citation

Dataset Description

Native dataset

Project Members

Name Additional Roles Affiliation While Working on the Project
Yong GengData CollectorColumbia University
Lidia MosyakColumbia University
Qing R FanData Collector, Depositor, PIColumbia University

Reprocessing Instructions

None

Reprocessing Data

About Reprocessing

Version:

version unreported

Reprocessing failed.

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97949

Sweep:

SWEEP1

Files:

/programs/datagrid/59/GBR214d_2OHsaclofen_1_###.img

Images:

1 - 180

Beam:

(157.48, 156.44) -> (157.48, 156.01)

Distance:

340.0 -> 341.47

Spacegroup:

P 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.16 5.86 2.16
Low resolution limit 44.52 44.52 2.2
Completeness 93.0 95.2 59.5
Multiplicity 2.0 1.9 2.0
I/sigma 8.8 18.2 1.1
Rmerge 0.045 0.036 0.364
CC half 0.98 0.972 0.505
Anomalous completeness 77.4 85.4 45.7
Anomalous multiplicity 1.1 1.1 1.1
Cell 70.31 73.21 112.81 90.02 90.08 97.78

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97949

Sweep:

SWEEP1

Files:

/programs/datagrid/59/GBR214d_2OHsaclofen_1_###.img

Images:

1 - 180

Beam:

(157.48, 156.44) -> (157.48, 156.04)

Distance:

340.0 -> 342.18

Spacegroup:

P 1 21 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.14 5.81 2.14
Low resolution limit 54.08 54.09 2.18
Completeness 96.5 96.0 74.6
Multiplicity 3.6 3.5 2.9
I/sigma 13.8 31.2 1.0
Rmerge 0.04 0.025 0.759
CC half 0.997 0.994 0.484
Anomalous completeness 92.8 88.3 70.5
Anomalous multiplicity 1.9 1.9 1.9
Cell 70.37 112.89 73.26 90.0 97.79 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97949

Sweep:

SWEEP1

Files:

/programs/datagrid/59/GBR214d_2OHsaclofen_1_###.img

Images:

1 - 180

Beam:

(157.48, 156.44) -> (157.48, 156.02)

Distance:

340.0 -> 343.12

Spacegroup:

P 1 21 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.98 5.37 1.98
Low resolution limit 37.61 37.62 2.01
Completeness 48.1 27.4 2.6
Multiplicity 2.6 1.5 1.1
I/sigma 10.3 11.4 1.0
Rmerge 0.035 0.023 0.326
CC half 0.997 0.996 0.707
Anomalous completeness 29.2 6.9 0.1
Anomalous multiplicity 1.7 1.7 1.7
Cell 70.363 112.829 73.263 90.0 97.773 90.0

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.