X-Ray Diffraction data from neoleukin-2/15 complex with IL-2R beta and gamma receptors, source of 6DG5 structure

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Data DOI: 10.15785/SBGRID/588 | ID: 588

Publication DOI: 10.1038/s41586-018-0830-7

6DG5 Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Garcia Laboratory, Stanford University

Release Date: 11 Jan 2019

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/588

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/588 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/588 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/588 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/588 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 588 ; shasum -c files.sha'

Storage requirements: 11G

Biological Sample:

neoleukin-2/15 complex with IL-2R beta and gamma receptors

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

ALS 5.0.1

Data Creation Date:

28 Jan 2018

Related Datasets:

None


Cite this Dataset

Jude, KM; Garcia, KC; Garcia, K. 2019. "X-Ray Diffraction data for: neoleukin-2/15 complex with IL-2R beta and gamma receptors. PDB Code 6DG5", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/588.

Download Citation

Dataset Description

native dataset, highly anisotropic

Project Members

Name Additional Roles Affiliation While Working on the Project
Kevin M JudeData Collector, DepositorStanford University/Howard Hughes Medical Institute
K C GarciaStanford University/Howard Hughes Medical Institute
K. Christopher GarciaPIStanford University

Reprocessing Instructions

First 1280 images were processed with XDS and scaled in aimless. Unmerged, scaled reflections were submitted to the STARANISO server for elliptical truncation

Reprocessing Data

About Reprocessing

xia2 -2d

xia2 -3d

xia2 -3dii

xia2 -dials
/programs/x86_64-linux/dials/3.12.0/bin.capsules//xia2 pipeline=3d /programs/datagrid//588/ verbose=True multiprocessing.nproc=1 atom=X

Version:

version unreported

Reprocessing failed.

/programs/x86_64-linux/dials/3.12.0/bin.capsules//xia2 pipeline=3dii /programs/datagrid//588/ verbose=True multiprocessing.nproc=1 atom=X

Version:

version unreported

Reprocessing failed.

/programs/x86_64-linux/dials/3.12.0/bin.capsules//xia2 pipeline=dials /programs/datagrid//588/ verbose=True multiprocessing.nproc=1 atom=X

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97741

Sweep:

SWEEP1

Files:

/programs/datagrid/588/1448-13_10_#####.cbf

Images:

1 - 1800

Beam:

(223.94, 237.53) -> (223.94, 236.95)

Distance:

500.0 -> 498.96

Spacegroup:

P 21 2 21

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.78 7.54 2.78
Low resolution limit 85.75 85.79 2.83
Completeness 99.9 100.0 94.2
Multiplicity 12.8 11.7 13.2
I/sigma 3.0 15.7 0.2
Rmerge 0.714 0.117 10.755
CC half 0.988 0.995 0.326
Anomalous completeness 99.7 100.0 94.0
Anomalous multiplicity 6.9 6.9 6.9
Cell 65.126 67.693 171.498 90.0 90.0 90.0

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.