X-Ray Diffraction data from single chain complex of viral US28 with stabilizing nanobody nb7, source of 5WB1 structure

Data DOI: 10.15785/SBGRID/581 | ID: 581

Publication DOI: 10.7554/eLife.35850

5WB1 Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Garcia Laboratory, Stanford University

Release Date: 8 Jun 2018

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/581

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/581 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/581 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/581 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/581 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 581 ; shasum -c files.sha'

Storage requirements: 11G

Biological Sample:

single chain complex of viral US28 with stabilizing nanobody nb7

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

APS 23 ID-D

Data Creation Date:

20 Apr 2015

Related Datasets:

None


Cite this Dataset

Jude, KM; Burg, JS; Garcia, K. 2018. "X-Ray Diffraction data for: single chain complex of viral US28 with stabilizing nanobody nb7. PDB Code 5WB1", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/581.

Download Citation

Dataset Description

merged data from 2 crystals

Project Members

Name Additional Roles Affiliation While Working on the Project
Kevin M JudeData Collector, DepositorStanford University/Howard Hughes Medical Institute
John S BurgData CollectorStanford University
K. Christopher GarciaPIStanford University

Reprocessing Instructions

datasets were processed separately with xds and merged with xscale

Reprocessing Data

About Reprocessing

Version:

version unreported

Reprocessing failed.

Version:

version unreported

Reprocessing failed.

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 1.0332

Sweep:

SWEEP2

Files:

/programs/datagrid/581/E2_1_#####.cbf

Images:

1 - 900

Beam:

(219.82, 218.44) -> (219.82, 219.61)

Distance:

650.0 -> 650.37

Spacegroup:

P 1 21 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 3.58 9.69 3.58
Low resolution limit 46.92 46.92 3.64
Completeness 99.5 100.0 99.4
Multiplicity 6.1 5.4 3.4
I/sigma 5.6 15.3 1.0
Rmerge 0.171 0.073 0.86
CC half 0.993 0.992 0.587
Anomalous completeness 98.7 99.6 95.3
Anomalous multiplicity 3.3 3.3 3.3
Cell 77.46 38.34 99.72 90.0 107.49 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 1.0332

Sweep:

SWEEP2

Files:

/programs/datagrid/581/E2_1_#####.cbf

Images:

1 - 900

Beam:

(219.82, 218.44) -> (219.82, 219.61)

Distance:

650.0 -> 649.37

Spacegroup:

P 1 21 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 3.37 9.14 3.37
Low resolution limit 69.21 69.23 3.43
Completeness 100.0 100.0 98.1
Multiplicity 6.4 5.8 6.1
I/sigma 6.2 15.2 2.1
Rmerge 0.174 0.072 0.64
CC half 0.988 0.985 0.795
Anomalous completeness 99.7 100.0 97.2
Anomalous multiplicity 3.4 3.4 3.4
Cell 77.39 38.275 99.58 90.0 107.457 90.0

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.