X-Ray Diffraction data from Human GABA(B) receptor extracellular domain in complex with the agonist baclofen, source of 4MS4 structure

Data DOI: 10.15785/SBGRID/58 | ID: 58

Publication DOI: 10.1038/nature12725

4MS4 Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Fan Laboratory, Columbia University

Release Date: May 7, 2015

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/58

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/58 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/58 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/58 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/58 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 58 ; shasum -c files.sha'

Storage requirements: 3.2G

Biological Sample:

Human GABA(B) receptor extracellular domain in complex with the agonist baclofen

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

Beamline 24-ID-C, NE-CAT, APS, Chicago, IL

Data Creation Date:

Aug. 15, 2011

Related Datasets:

None


Cite this Dataset

Geng, Y; Mosyak, L; Fan, QR. 2015. "X-Ray Diffraction data for: Human GABA(B) receptor extracellular domain in complex with the agonist baclofen. PDB Code 4MS4", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/58.

Download Citation

Dataset Description

Native dataset

Project Members

Name Additional Roles Affiliation While Working on the Project
Yong GengData CollectorColumbia University
Lidia MosyakColumbia University
Qing R FanData Collector, Depositor, PIColumbia University

Reprocessing Instructions

None

Reprocessing Data

About Reprocessing

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97949

Sweep:

SWEEP1

Files:

/programs/datagrid/58/baclofen7a_1_###.img

Images:

1 - 180

Beam:

(157.96, 156.52) -> (157.96, 156.17)

Distance:

250.0 -> 251.22

Spacegroup:

P 2 2 21

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.75 4.75 1.75
Low resolution limit 59.37 59.4 1.78
Completeness 96.9 98.9 84.7
Multiplicity 6.9 6.9 6.6
I/sigma 7.4 16.3 1.1
Rmerge 0.171 0.113 1.845
CC half 0.981 0.961 0.315
Anomalous completeness 95.0 99.3 80.9
Anomalous multiplicity 3.6 3.6 3.6
Cell 59.365 115.246 140.315 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97949

Sweep:

SWEEP1

Files:

/programs/datagrid/58/baclofen7a_1_###.img

Images:

1 - 180

Beam:

(157.96, 156.52) -> (157.96, 156.1)

Distance:

250.0 -> 251.2

Spacegroup:

P 2 2 21

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.77 4.8 1.77
Low resolution limit 35.65 35.65 1.8
Completeness 98.1 99.2 96.1
Multiplicity 7.4 6.8 7.6
I/sigma 14.8 43.9 1.0
Rmerge 0.078 0.028 1.957
CC half 0.999 0.998 0.389
Anomalous completeness 98.1 99.8 96.4
Anomalous multiplicity 3.9 3.9 3.9
Cell 59.43 115.31 140.36 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97949

Sweep:

SWEEP1

Files:

/programs/datagrid/58/baclofen7a_1_###.img

Images:

1 - 180

Beam:

(157.96, 156.52) -> (157.96, 156.1)

Distance:

250.0 -> 251.24

Spacegroup:

P 2 2 21

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.76 4.78 1.76
Low resolution limit 54.73 54.75 1.79
Completeness 97.9 99.1 93.5
Multiplicity 7.4 6.8 7.5
I/sigma 14.6 43.9 1.0
Rmerge 0.079 0.028 2.078
CC half 0.999 0.999 0.354
Anomalous completeness 97.9 99.7 93.7
Anomalous multiplicity 3.9 3.9 3.9
Cell 59.43 115.31 140.36 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97949

Sweep:

SWEEP1

Files:

/programs/datagrid/58/baclofen7a_1_###.img

Images:

1 - 180

Beam:

(157.96, 156.52) -> (157.96, 156.09)

Distance:

250.0 -> 251.04

Spacegroup:

P 2 2 21

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.88 5.1 1.88
Low resolution limit 53.35 53.37 1.91
Completeness 87.9 99.3 47.5
Multiplicity 5.5 7.0 2.9
I/sigma 14.7 37.1 0.7
Rmerge 0.059 0.031 1.587
CC half 0.999 0.999 0.291
Anomalous completeness 84.5 99.8 33.5
Anomalous multiplicity 2.9 2.9 2.9
Cell 59.454 115.349 140.344 90.0 90.0 90.0

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.