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X-Ray Diffraction data from Deinococcus radiodurans Nramp/MntH divalent transition metal transporter mutant G45R, source of 6C3I structure

Data DOI: 10.15785/SBGRID/567 | ID: 567

Publication DOI: 10.7554/eLife.41124

6C3I Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Gaudet Laboratory, Harvard University

Release Date: Feb. 5, 2019

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/567

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/567 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/567 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/567 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/567 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 567 ; shasum -c files.sha'

Storage requirements: 3.8G

Biological Sample:

Deinococcus radiodurans Nramp/MntH divalent transition metal transporter mutant G45R

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

24-ID-C, NE-CAT, APS, Chicago, IL

Data Creation Date:

July 21, 2016

Related Datasets:

None


Cite this Dataset

Bozzi, AT; Zimanyi, CM; Gaudet, R. 2019. "X-Ray Diffraction data for: Deinococcus radiodurans Nramp/MntH divalent transition metal transporter mutant G45R. PDB Code 6C3I", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/567.

Download Citation

Dataset Description

Two wedges from separate LCP crystals were combined for a final data set that consisted of frames 1-400 of RG007_02_2 and frames 1-150 of RG007_03_1.

Project Members

Name Additional Roles Affiliation While Working on the Project
Aaron T BozziDepositorHarvard University
Christina M ZimanyiData CollectorHarvard University
Rachelle Gaudet, PIHarvard University

Reprocessing Instructions

The data was processed in space group P212121 with resulting unit cell dimensions of 70.7, 97.9, and 118.3 Angstroms.

Reprocessing Data

About Reprocessing

Version:

version unreported

Reprocessing failed.

Version:

version unreported

Reprocessing failed.

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.9793

Sweep:

SWEEP2

Files:

/programs/datagrid/567/RG007_03_1_1_####.cbf

Images:

1 - 200

Beam:

(216.33, 222.38) -> (216.33, 222.33)

Distance:

450.0 -> 451.52

Spacegroup:

P 1 21 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 3.04 8.25 3.04
Low resolution limit 70.85 70.87 3.09
Completeness 86.9 83.1 84.6
Multiplicity 2.3 2.6 1.8
I/sigma 2.7 15.2 -0.4
Rmerge 0.358 0.234 1.241
CC half 0.839 0.896 0.344
Anomalous completeness 59.7 70.2 45.6
Anomalous multiplicity 1.4 1.4 1.4
Cell 59.19 98.96 70.85 90.0 90.05 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.9793

Sweep:

SWEEP2

Files:

/programs/datagrid/567/RG007_03_1_1_####.cbf

Images:

1 - 200

Beam:

(216.33, 222.38) -> (216.33, 222.33)

Distance:

450.0 -> 452.33

Spacegroup:

P 1 2 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 4.09 11.08 4.09
Low resolution limit 70.99 71.0 4.16
Completeness 91.0 90.0 74.1
Multiplicity 2.1 2.4 1.9
I/sigma 8.6 14.1 6.1
Rmerge 0.088 0.034 0.137
CC half 0.995 0.999 0.979
Anomalous completeness 70.1 81.9 54.5
Anomalous multiplicity 1.3 1.3 1.3
Cell 70.996 59.243 98.744 90.0 90.517 90.0

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.