X-Ray Diffraction data from RSV F in complex with 5C4 Fab, source of 5W23 structure

Data DOI: 10.15785/SBGRID/536 | ID: 536

Publication DOI: 10.1038/s41467-017-01858-w

5W23 Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

McLellan Laboratory, University of Texas at Austin

Release Date: Dec. 26, 2017

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/536

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/536 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/536 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/536 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/536 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 536 ; shasum -c files.sha'

Storage requirements: 5.7G

Biological Sample:

RSV F in complex with 5C4 Fab

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

CHESS beamline A1 (Cornell High Energy Synchrotron Source)

Data Creation Date:

June 6, 2014

Related Datasets:

None


Cite this Dataset

Battles, MB; McLellan, J. 2017. "X-Ray Diffraction data for: RSV F in complex with 5C4 Fab. PDB Code 5W23", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/536.

Download Citation

Dataset Description

Native dataset

Project Members

Name Additional Roles Affiliation While Working on the Project
Michael B BattlesData Collector, DepositorGeisel School of Medicine at Dartmouth
Jason McLellanPIUniversity of Texas at Austin

Reprocessing Instructions

1) Reverse spindle rotation for goniometer 2) Error with detector distance (297.3, not 300mm) 3) Process in P4(1)2(1)2 4) Molecular replacement using high resolution RSV F (PDB ID: 5C69) and 5C4 Fab (PDB ID:5W24)

Reprocessing Data

About Reprocessing

Version:

version unreported

Reprocessing failed.

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.977

Sweep:

SWEEP1

Files:

/programs/datagrid/536/Fw5C4p5p5_1_###.img

Images:

1 - 180

Beam:

(107.98, 108.7) -> (107.98, 108.68)

Distance:

300.0 -> 307.3

Spacegroup:

P 1 2 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 5.48 14.59 5.48
Low resolution limit 37.39 37.39 5.57
Completeness 67.1 63.5 67.2
Multiplicity 2.5 2.3 2.4
I/sigma 0.3 0.2 0.3
Rmerge 1.306 0.981 1.972
CC half 0.012 0.086 0.006
Anomalous completeness 53.5 52.0 52.6
Anomalous multiplicity 1.4 1.4 1.4
Cell 192.34 184.1 282.85 90.0 94.74 90.0

Version:

version unreported

Reprocessing failed.

Version:

version unreported

Reprocessing failed.


License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.