X-Ray Diffraction data from Human metapneumovirus fusion glycoprotein stabilized in the prefusion state, source of 5WB0 structure

Data DOI: 10.15785/SBGRID/535 | ID: 535

Publication DOI: 10.1038/s41467-017-01708-9

5WB0 Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

McLellan Laboratory, University of Texas at Austin

Release Date: 9 Jan 2018

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/535

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/535 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/535 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/535 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/535 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 535 ; shasum -c files.sha'

Storage requirements: 4.3G

Biological Sample:

Human metapneumovirus fusion glycoprotein stabilized in the prefusion state

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

AMX 17-ID-1 beamline (NSLS II, Brookhaven National Laboratory)

Data Creation Date:

7 Apr 2017

Related Datasets:

None


Cite this Dataset

Battles, MB; McLellan, J. 2018. "X-Ray Diffraction data for: Human metapneumovirus fusion glycoprotein stabilized in the prefusion state. PDB Code 5WB0", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/535.

Download Citation

Dataset Description

Native dataset

Project Members

Name Additional Roles Affiliation While Working on the Project
Michael B BattlesData Collector, DepositorGeisel School of Medicine at Dartmouth
Jason McLellanPIUniversity of Texas at Austin

Reprocessing Instructions

1) Convert image files using eiger2cbf 2) Beam center is: x beam 115.54 y beam 113.38 3) Use 440 frames (11-450) 4) Indexed in space group I2(1)3 5) Molecular replacement with a hybrid search model consisting of domain II of the post-fusion hMPV F structure (PDB ID: 5L1X) and a high-resolution structure of pre-fusion hRSV F (PDB ID: 5C69)

Reprocessing Data

About Reprocessing

Version:

version unreported

Reprocessing failed.

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.91977

Sweep:

SWEEP1

Files:

/programs/datagrid/535/out######.cbf

Images:

1 - 450

Beam:

(116.62, 113.17) -> (116.62, 113.24)

Distance:

300.0 -> 300.52

Spacegroup:

I 2 3

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.68 7.27 2.68
Low resolution limit 56.31 56.32 2.73
Completeness 100.0 99.7 99.9
Multiplicity 10.4 9.8 10.9
I/sigma 14.7 42.8 1.5
Rmerge 0.119 0.038 1.643
CC half 0.999 0.999 0.557
Anomalous completeness 99.9 99.9 99.9
Anomalous multiplicity 5.4 5.4 5.4
Cell 178.07 178.07 178.07 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.91977

Sweep:

SWEEP1

Files:

/programs/datagrid/535/out######.cbf

Images:

1 - 450

Beam:

(116.62, 113.17) -> (116.62, 113.24)

Distance:

300.0 -> 300.14

Spacegroup:

I 2 3

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.67 7.25 2.67
Low resolution limit 56.31 56.32 2.72
Completeness 100.0 99.7 99.8
Multiplicity 10.4 9.8 10.9
I/sigma 14.6 42.7 1.4
Rmerge 0.119 0.038 1.664
CC half 0.999 0.999 0.53
Anomalous completeness 99.9 99.9 99.9
Anomalous multiplicity 5.4 5.4 5.4
Cell 178.07 178.07 178.07 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.91977

Sweep:

SWEEP1

Files:

/programs/datagrid/535/out######.cbf

Images:

1 - 450

Beam:

(116.62, 113.17) -> (116.62, 113.24)

Distance:

300.0 -> 300.47

Spacegroup:

I 2 3

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.55 6.92 2.55
Low resolution limit 56.31 56.33 2.59
Completeness 100.0 100.0 100.0
Multiplicity 10.4 10.0 10.8
I/sigma 12.0 32.1 2.3
Rmerge 0.123 0.046 0.814
CC half 0.998 0.999 0.481
Anomalous completeness 100.0 100.0 100.0
Anomalous multiplicity 5.4 5.4 5.4
Cell 178.073 178.073 178.073 90.0 90.0 90.0

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.