X-Ray Diffraction data from Kluyveromyces lactis Ctf19-Mcm21, source of 3ZXU structure

Data DOI: 10.15785/SBGRID/522 | ID: 522

Publication DOI: 10.1038/embor.2012.1

3ZXU Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Harrison Laboratory, Harvard Medical School

Release Date: Nov. 21, 2017

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/522

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/522 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/522 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/522 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/522 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 522 ; shasum -c files.sha'

Storage requirements: 2.5G

Biological Sample:

Kluyveromyces lactis Ctf19-Mcm21

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

24-ID-C at Advanced Photon Source, Argonne National Laboratory

Data Creation Date:

Aug. 18, 2010

Related Datasets:

523


Cite this Dataset

Schmitzberger, F; Harrison, S. 2017. "X-Ray Diffraction data for: Kluyveromyces lactis Ctf19-Mcm21. PDB Code 3ZXU", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/522.

Download Citation

Dataset Description

X-ray diffraction data of crystals from Kluyveromyces lactis central kinetochore proteins Ctf19-Mcm21; one anomalous data set from selenomethionine-derivatized protein crystal;

Project Members

Name Additional Roles Affiliation While Working on the Project
Florian SchmitzbergerData Collector, DepositorHarvard Medical School
Stephen HarrisonPIHarvard Medical School

Reprocessing Instructions

none

Reprocessing Data

About Reprocessing

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97912

Sweep:

SWEEP1

Files:

/programs/datagrid/522/Puck4_Crys13_2/Crys13_2_1_###.img

Images:

1 - 140

Beam:

(156.71, 156.32) -> (156.71, 156.25)

Distance:

500.0 -> 501.7

Spacegroup:

P 62 2 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 5.86 15.89 5.86
Low resolution limit 103.97 103.99 5.96
Completeness 100.0 100.0 100.0
Multiplicity 15.5 12.4 16.6
I/sigma 20.0 36.4 5.3
Rmerge 0.095 0.036 0.673
CC half 0.998 0.997 0.926
Anomalous completeness 100.0 99.3 100.0
Anomalous multiplicity 8.6 8.6 8.6
Cell 240.113 240.113 179.344 90.0 90.0 120.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97912

Sweep:

SWEEP1

Files:

/programs/datagrid/522/Puck4_Crys13_2/Crys13_2_1_###.img

Images:

1 - 140

Beam:

(156.71, 156.32) -> (156.71, 156.2)

Distance:

500.0 -> 499.69

Spacegroup:

P 62 2 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 6.11 16.47 6.11
Low resolution limit 58.96 58.96 6.22
Completeness 100.0 99.1 100.0
Multiplicity 15.9 11.6 17.0
I/sigma 29.9 54.2 6.6
Rmerge 0.069 0.031 0.549
CC half 0.999 0.999 0.985
Anomalous completeness 99.9 99.2 100.0
Anomalous multiplicity 8.9 8.9 8.9
Cell 239.53 239.53 178.87 90.0 90.0 120.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97912

Sweep:

SWEEP1

Files:

/programs/datagrid/522/Puck4_Crys13_2/Crys13_2_1_###.img

Images:

1 - 140

Beam:

(156.71, 156.32) -> (156.71, 156.2)

Distance:

500.0 -> 500.84

Spacegroup:

P 62 2 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 6.2 16.7 6.2
Low resolution limit 57.54 57.54 6.31
Completeness 100.0 99.1 100.0
Multiplicity 15.9 11.5 16.6
I/sigma 30.9 53.9 7.3
Rmerge 0.067 0.031 0.51
CC half 0.999 0.999 0.991
Anomalous completeness 99.8 99.1 99.7
Anomalous multiplicity 8.8 8.8 8.8
Cell 239.54 239.54 178.87 90.0 90.0 120.0

Version:

version unreported

Reprocessing failed.


License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.