X-Ray Diffraction data from Kluyveromyces lactis Ctf19-Mcm21 D-RWD domains bound with fragment of Kluyveromyces lactis Okp1, source of 5MU3 structure

Data DOI: 10.15785/SBGRID/521 | ID: 521

Publication DOI: 10.15252/embj.201796636

5MU3 Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Harrison Laboratory, Harvard Medical School

Release Date: Nov. 21, 2017

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/521

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/521 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/521 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/521 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/521 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 521 ; shasum -c files.sha'

Storage requirements: 2.3G

Biological Sample:

Kluyveromyces lactis Ctf19-Mcm21 D-RWD domains bound with fragment of Kluyveromyces lactis Okp1

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

24-ID-E at Advanced Photon Source, Argonne National Laboratory

Data Creation Date:

July 10, 2014

Related Datasets:

None


Cite this Dataset

Schmitzberger, F; Harrison, S. 2017. "X-Ray Diffraction data for: Kluyveromyces lactis Ctf19-Mcm21 D-RWD domains bound with fragment of Kluyveromyces lactis Okp1. PDB Code 5MU3", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/521.

Download Citation

Dataset Description

X-ray diffraction data of crystals of Kluyveromyces lactis central kinetochore proteins Ctf19-Mcm21 D-RWD domains bound with fragment of Kluyveromyces lactis central kinetochore protein Okp1

Project Members

Name Additional Roles Affiliation While Working on the Project
Florian SchmitzbergerData Collector, DepositorHarvard Medical School, Research Institute of Molecular Pathology (IMP, Vienna), University of Duisburg-Essen
Stephen HarrisonPIHarvard Medical School

Reprocessing Instructions

none

Reprocessing Data

About Reprocessing

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97918

Sweep:

SWEEP1

Files:

/programs/datagrid/521/scb007_crys07_1_1_###.img

Images:

1 - 130

Beam:

(152.88, 158.86) -> (152.88, 159.01)

Distance:

292.0 -> 291.9

Spacegroup:

P 21 21 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.12 5.75 2.12
Low resolution limit 60.83 60.85 2.16
Completeness 99.8 98.7 99.7
Multiplicity 5.1 4.8 5.2
I/sigma 8.1 20.8 1.1
Rmerge 0.11 0.04 1.385
CC half 0.996 0.997 0.365
Anomalous completeness 99.3 97.8 98.9
Anomalous multiplicity 2.7 2.7 2.7
Cell 105.582 123.124 93.414 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97918

Sweep:

SWEEP1

Files:

/programs/datagrid/521/scb007_crys07_1_1_###.img

Images:

1 - 130

Beam:

(152.88, 158.86) -> (152.88, 158.96)

Distance:

292.0 -> 291.57

Spacegroup:

P 21 21 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.22 6.02 2.22
Low resolution limit 60.85 60.87 2.26
Completeness 99.9 98.4 100.0
Multiplicity 5.3 4.8 5.4
I/sigma 12.2 33.5 1.1
Rmerge 0.067 0.028 1.619
CC half 0.999 0.999 0.495
Anomalous completeness 98.6 97.1 99.0
Anomalous multiplicity 2.8 2.8 2.8
Cell 105.64 123.13 93.45 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97918

Sweep:

SWEEP1

Files:

/programs/datagrid/521/scb007_crys07_1_1_###.img

Images:

1 - 130

Beam:

(152.88, 158.86) -> (152.88, 158.96)

Distance:

292.0 -> 291.63

Spacegroup:

P 21 21 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.23 6.05 2.23
Low resolution limit 60.85 60.87 2.27
Completeness 99.9 98.4 99.9
Multiplicity 5.3 4.8 5.4
I/sigma 12.3 33.5 1.2
Rmerge 0.067 0.028 1.484
CC half 0.999 0.999 0.555
Anomalous completeness 98.6 97.1 99.0
Anomalous multiplicity 2.8 2.8 2.8
Cell 105.64 123.13 93.45 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97918

Sweep:

SWEEP1

Files:

/programs/datagrid/521/scb007_crys07_1_1_###.img

Images:

1 - 130

Beam:

(152.88, 158.86) -> (152.88, 158.94)

Distance:

292.0 -> 290.92

Spacegroup:

P 21 21 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.18 5.91 2.18
Low resolution limit 105.3 105.42 2.21
Completeness 99.6 98.5 91.7
Multiplicity 5.3 4.9 5.4
I/sigma 9.7 24.4 1.2
Rmerge 0.087 0.036 1.316
CC half 0.998 0.998 0.604
Anomalous completeness 99.4 98.0 91.5
Anomalous multiplicity 2.7 2.7 2.7
Cell 105.301 122.948 93.331 90.0 90.0 90.0

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.