X-Ray Diffraction data from Mycobacterial glucosylglycerate hydrolase - E419A mutant in complex with mannosylglycerate, source of 5OJV structure

Data DOI: 10.15785/SBGRID/475 | ID: 475

Publication DOI: 10.1107/S2052252519005372

5OJV Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Pereira Laboratory, IBMC/i3S, Universidade do Porto

Release Date: May 3, 2019

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/475

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/475 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/475 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/475 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/475 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 475 ; shasum -c files.sha'

Storage requirements: 21G

Biological Sample:

Mycobacterial glucosylglycerate hydrolase - E419A mutant in complex with mannosylglycerate

Dataset Type:

X-Ray Diffraction

Subject Composition:

Ligand, Protein

Collection Facility:

ESRF ID30B

Data Creation Date:

July 24, 2016

Related Datasets:

None


Cite this Dataset

Cereija, TB.; Pereira, PJ.B.. 2019. "X-Ray Diffraction data for: Mycobacterial glucosylglycerate hydrolase - E419A mutant in complex with mannosylglycerate. PDB Code 5OJV", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/475.

Download Citation

Dataset Description

X-Ray Diffraction data from M. hassiacum GgH, source of 5OJV structure

Project Members

Name Additional Roles Affiliation While Working on the Project
Pedro J.B. PereiraDepositorIBMC - Instituto de Biologia Molecular e Celular
Tatiana B. CereijaData CollectorIBMC - Instituto de Biologia Molecular e Celular
Pedro PereiraPIUniversidade do Porto

Reprocessing Instructions

none

Reprocessing Data

About Reprocessing

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97625

Sweep:

SWEEP1

Files:

/programs/datagrid/475/bg3047_w1_1_####.cbf

Images:

1 - 3600

Beam:

(217.25, 209.45) -> (217.25, 209.79)

Distance:

317.93 -> 317.86

Spacegroup:

P 1 2 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.98 5.37 1.98
Low resolution limit 87.39 87.47 2.01
Completeness 96.6 96.2 97.3
Multiplicity 3.3 3.3 3.4
I/sigma 6.9 16.9 1.6
Rmerge 0.089 0.037 0.667
CC half 0.992 0.993 0.642
Anomalous completeness 88.6 91.3 89.0
Anomalous multiplicity 1.7 1.7 1.7
Cell 87.481 87.387 157.333 90.0 90.421 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97625

Sweep:

SWEEP1

Files:

/programs/datagrid/475/bg3047_w1_1_####.cbf

Images:

1 - 3600

Beam:

(217.25, 209.45) -> (217.25, 209.7)

Distance:

317.93 -> 317.95

Spacegroup:

P 21 21 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.35 6.37 2.35
Low resolution limit 39.17 39.17 2.39
Completeness 98.6 96.7 99.5
Multiplicity 6.3 5.8 6.6
I/sigma 16.9 32.7 4.3
Rmerge 0.06 0.031 0.405
CC half 0.998 0.999 0.92
Anomalous completeness 95.9 95.1 98.4
Anomalous multiplicity 3.3 3.3 3.3
Cell 87.75 158.17 87.54 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97625

Sweep:

SWEEP1

Files:

/programs/datagrid/475/bg3047_w1_1_####.cbf

Images:

1 - 3600

Beam:

(217.25, 209.45) -> (217.25, 209.69)

Distance:

317.93 -> 317.59

Spacegroup:

P 21 21 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.01 5.46 2.01
Low resolution limit 76.73 76.79 2.04
Completeness 98.7 97.8 99.4
Multiplicity 6.3 5.9 6.6
I/sigma 11.4 31.1 1.0
Rmerge 0.076 0.034 1.651
CC half 0.998 0.998 0.625
Anomalous completeness 96.6 95.8 99.1
Anomalous multiplicity 3.3 3.3 3.3
Cell 87.75 158.17 87.54 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97625

Sweep:

SWEEP1

Files:

/programs/datagrid/475/bg3047_w1_1_####.cbf

Images:

1 - 3600

Beam:

(217.25, 209.45) -> (217.25, 209.68)

Distance:

317.93 -> 318.29

Spacegroup:

P 21 21 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.98 5.37 1.98
Low resolution limit 87.54 87.63 2.01
Completeness 98.7 98.1 97.2
Multiplicity 6.6 6.3 6.7
I/sigma 7.2 17.0 1.1
Rmerge 0.119 0.042 1.442
CC half 0.996 0.996 0.879
Anomalous completeness 98.4 97.7 96.8
Anomalous multiplicity 3.4 3.4 3.4
Cell 87.702 158.026 87.544 90.0 90.0 90.0

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.