X-Ray Diffraction data from Mycobacterial glucosylglycerate hydrolase - E419A mutant, source of 5OIE structure

Data DOI: 10.15785/SBGRID/470 | ID: 470

Publication DOI: 10.1107/S2052252519005372

5OIE Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Pereira Laboratory, IBMC/i3S, Universidade do Porto

Release Date: May 3, 2019

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/470

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/470 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/470 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/470 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/470 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 470 ; shasum -c files.sha'

Storage requirements: 1.5G

Biological Sample:

Mycobacterial glucosylglycerate hydrolase - E419A mutant

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

ESRF ID30A1

Data Creation Date:

Dec. 12, 2015

Related Datasets:

None


Cite this Dataset

Cereija, TB.; Pereira, PJ.B.. 2019. "X-Ray Diffraction data for: Mycobacterial glucosylglycerate hydrolase - E419A mutant. PDB Code 5OIE", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/470.

Download Citation

Dataset Description

X-Ray Diffraction data from M. hassiacum GgH, source of 5OIE structure

Project Members

Name Additional Roles Affiliation While Working on the Project
Pedro J.B. PereiraDepositorIBMC - Instituto de Biologia Molecular e Celular
Tatiana B. CereijaData CollectorIBMC - Instituto de Biologia Molecular e Celular
Pedro PereiraPIUniversidade do Porto

Reprocessing Instructions

none

Reprocessing Data

About Reprocessing

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.966

Sweep:

SWEEP1

Files:

/programs/datagrid/470/GGH01-GGH16_1_####.cbf

Images:

1 - 640

Beam:

(146.86, 129.01) -> (146.86, 129.18)

Distance:

225.0 -> 224.5

Spacegroup:

P 21 21 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.14 5.81 2.14
Low resolution limit 61.76 61.79 2.18
Completeness 99.4 99.8 99.7
Multiplicity 4.3 4.5 3.8
I/sigma 7.0 12.8 2.2
Rmerge 0.134 0.064 1.079
CC half 0.989 0.992 0.327
Anomalous completeness 91.2 99.2 92.8
Anomalous multiplicity 2.3 2.3 2.3
Cell 159.249 86.343 88.382 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.966

Sweep:

SWEEP1

Files:

/programs/datagrid/470/GGH01-GGH16_1_####.cbf

Images:

1 - 640

Beam:

(146.86, 129.01) -> (146.86, 129.05)

Distance:

225.0 -> 223.76

Spacegroup:

P 21 21 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.96 5.32 1.96
Low resolution limit 48.8 48.82 1.99
Completeness 99.2 97.8 99.6
Multiplicity 4.5 4.2 4.8
I/sigma 10.5 28.6 1.1
Rmerge 0.078 0.034 1.77
CC half 0.997 0.998 0.473
Anomalous completeness 92.7 89.8 95.9
Anomalous multiplicity 2.4 2.4 2.4
Cell 159.44 86.22 88.38 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.966

Sweep:

SWEEP1

Files:

/programs/datagrid/470/GGH01-GGH16_1_####.cbf

Images:

1 - 640

Beam:

(146.86, 129.01) -> (146.86, 129.06)

Distance:

225.0 -> 224.2

Spacegroup:

P 21 21 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.93 5.24 1.93
Low resolution limit 57.55 57.58 1.96
Completeness 99.2 97.7 99.8
Multiplicity 4.5 4.1 4.8
I/sigma 10.1 28.5 0.9
Rmerge 0.079 0.034 2.081
CC half 0.997 0.998 0.33
Anomalous completeness 92.8 89.3 95.9
Anomalous multiplicity 2.4 2.4 2.4
Cell 159.44 86.22 88.38 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.966

Sweep:

SWEEP1

Files:

/programs/datagrid/470/GGH01-GGH16_1_####.cbf

Images:

1 - 640

Beam:

(146.86, 129.01) -> (146.86, 129.06)

Distance:

225.0 -> 224.5

Spacegroup:

P 21 21 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.11 5.72 2.11
Low resolution limit 75.85 75.9 2.14
Completeness 99.3 99.8 93.6
Multiplicity 4.5 4.6 4.1
I/sigma 6.0 10.7 2.0
Rmerge 0.129 0.057 1.341
CC half 0.992 0.995 0.21
Anomalous completeness 93.8 99.2 88.6
Anomalous multiplicity 2.4 2.4 2.4
Cell 159.213 86.264 88.221 90.0 90.0 90.0

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.