X-Ray Diffraction data from SpoIIE from Bacillus subtilis, source of 5UCG structure

Data DOI: 10.15785/SBGRID/469 | ID: 469

Publication DOI: 10.7554/eLife.26111

5UCG Coordinates: Viewer, PDB, MMDB

Gaudet Laboratory, Harvard University

Release Date: 21 Jul 2017

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/469

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/469 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/469 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/469 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/469 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 469 ; shasum -c files.sha'

Storage requirements: 5.3G

Biological Sample:

SpoIIE from Bacillus subtilis

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

24-ID-C, NE-CAT, APS, Chicago, IL.

Data Creation Date:

18 Feb 2016

Related Datasets:

None


Cite this Dataset

Bradshaw, N; Zimanyi, CM; Gaudet, R. 2017. "X-Ray Diffraction data for: SpoIIE from Bacillus subtilis. PDB Code 5UCG", SBGrid Data Bank, V1, http://dx.doi.org/10.15785/SBGRID/469.

Download Citation

Dataset Description

Original images for 5UCG: Structure of the PP2C Phosphatase Domain and a Fragment of the Regulatory Domain of the Cell Fate Determinant SpoIIE from Bacillus Subtilis. From Bradshaw et al. eLife 2017;6:e26111.

Project Members

Name Additional Roles Affiliation While Working on the Project
Niels BradshawData Collector, DepositorHarvard University
Christina M ZimanyiData CollectorHarvard University
Rachelle GaudetData Collector, PIHarvard University

Reprocessing Instructions

none

Reprocessing Data

About Reprocessing

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.9792

Sweep:

SWEEP2

Files:

/programs/datagrid/469/RG002_07_2_####.cbf

Images:

1 - 450

Beam:

(216.5, 222.42) -> (216.5, 222.56)

Distance:

600.0 -> 602.9

Spacegroup:

P 41 21 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 3.99 10.83 3.99
Low resolution limit 125.68 125.74 4.06
Completeness 100.0 100.0 100.0
Multiplicity 11.8 11.8 11.8
I/sigma 8.3 8.3 8.3
Rmerge 0.122 0.122 0.122
CC half 0.997 0.997 0.997
Anomalous completeness 100.0 100.0 100.0
Anomalous multiplicity 6.4 6.4 6.4
Cell 125.68 125.68 330.634 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.9792

Sweep:

SWEEP2

Files:

/programs/datagrid/469/RG002_07_2_####.cbf

Images:

1 - 450

Beam:

(216.5, 222.42) -> (216.5, 222.46)

Distance:

600.0 -> 602.73

Spacegroup:

P 41 21 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 4.23 11.47 4.23
Low resolution limit 69.28 69.29 4.3
Completeness 100.0 100.0 100.0
Multiplicity 12.9 12.9 12.9
I/sigma 15.8 15.8 15.8
Rmerge 0.079 0.079 0.079
CC half 1.0 1.0 1.0
Anomalous completeness 100.0 100.0 100.0
Anomalous multiplicity 7.0 7.0 7.0
Cell 125.84 125.84 331.16 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.9792

Sweep:

SWEEP2

Files:

/programs/datagrid/469/RG002_07_2_####.cbf

Images:

1 - 450

Beam:

(216.5, 222.42) -> (216.5, 222.46)

Distance:

600.0 -> 602.24

Spacegroup:

P 41 21 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 4.24 11.51 4.24
Low resolution limit 117.63 117.68 4.31
Completeness 100.0 100.0 100.0
Multiplicity 13.0 13.0 13.0
I/sigma 15.9 15.9 15.9
Rmerge 0.078 0.078 0.078
CC half 1.0 1.0 1.0
Anomalous completeness 100.0 100.0 100.0
Anomalous multiplicity 7.1 7.1 7.1
Cell 125.84 125.84 331.17 90.0 90.0 90.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.9792

Sweep:

SWEEP2

Files:

/programs/datagrid/469/RG002_07_2_####.cbf

Images:

1 - 450

Beam:

(216.5, 222.42) -> (216.5, 222.46)

Distance:

600.0 -> 602.43

Spacegroup:

P 41 21 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 3.83 10.39 3.83
Low resolution limit 125.8 125.87 3.89
Completeness 100.0 100.0 100.0
Multiplicity 12.4 12.4 12.4
I/sigma 10.8 10.8 10.8
Rmerge 0.102 0.102 0.102
CC half 1.0 1.0 1.0
Anomalous completeness 99.9 99.9 99.9
Anomalous multiplicity 6.7 6.7 6.7
Cell 125.798 125.798 331.012 90.0 90.0 90.0

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.