X-Ray Diffraction data from E. Coli Alkaline Phosphatase in complex with tungstate, source of 5C66 structure

Data DOI: 10.15785/SBGRID/456 | ID: 456

Publication DOI: 10.1016/j.jmb.2016.05.007

5C66 Coordinates: Viewer, PDB, MMDB

Herschlag Laboratory, Stanford University

Release Date: 7 Jul 2017

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/456

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/456 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/456 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/456 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/456 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 456 ; shasum -c files.sha'

Storage requirements: 2.1G

Biological Sample:

E. Coli Alkaline Phosphatase in complex with tungstate

Dataset Type:

X-Ray Diffraction

Subject Composition:

Ligand, Protein

Collection Facility:

SSRL, BL12-2

Data Creation Date:

7 Feb 2013

Related Datasets:

None


Cite this Dataset

Peck, A; Ressl, S; Herschlag, D. 2017. "X-Ray Diffraction data for: E. Coli Alkaline Phosphatase in complex with tungstate. PDB Code 5C66", SBGrid Data Bank, V1, http://dx.doi.org/10.15785/SBGRID/456.

Download Citation

Dataset Description

Native dataset

Project Members

Name Additional Roles Affiliation While Working on the Project
Ariana PeckDepositorData Depositor
Susanne ResslData CollectorData Collector
Daniel HerschlagPIStanford University

Reprocessing Instructions

Data have successfully been processed using MOSFLM and XDS.

Reprocessing Data

About Reprocessing

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.9795

Sweep:

SWEEP1

Files:

/programs/datagrid/456/I3_1_#####.cbf

Images:

1 - 357

Beam:

(217.32, 211.82) -> (217.32, 211.83)

Distance:

400.0 -> 399.9

Spacegroup:

P 63 2 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.88 5.1 1.88
Low resolution limit 98.69 98.83 1.91
Completeness 91.5 89.2 79.7
Multiplicity 5.4 5.6 3.5
I/sigma 12.1 32.1 1.3
Rmerge 0.063 0.021 0.736
CC half 0.384 0.999 -0.018
Anomalous completeness 81.6 81.4 68.9
Anomalous multiplicity 3.0 3.0 3.0
Cell 161.179 161.179 139.549 90.0 90.0 120.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.9795

Sweep:

SWEEP1

Files:

/programs/datagrid/456/I3_1_#####.cbf

Images:

1 - 357

Beam:

(217.32, 211.82) -> (217.32, 211.73)

Distance:

400.0 -> 400.36

Spacegroup:

P 63 2 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.92 5.21 1.92
Low resolution limit 49.45 49.46 1.95
Completeness 92.5 90.0 84.9
Multiplicity 6.1 6.0 4.8
I/sigma 21.5 77.7 1.3
Rmerge 0.039 0.013 0.808
CC half 1.0 1.0 0.794
Anomalous completeness 85.1 82.7 79.8
Anomalous multiplicity 3.3 3.3 3.3
Cell 161.51 161.51 139.73 90.0 90.0 120.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.9795

Sweep:

SWEEP1

Files:

/programs/datagrid/456/I3_1_#####.cbf

Images:

1 - 357

Beam:

(217.32, 211.82) -> (217.32, 211.73)

Distance:

400.0 -> 400.31

Spacegroup:

P 63 2 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.91 5.18 1.91
Low resolution limit 49.45 49.46 1.94
Completeness 92.4 90.0 83.3
Multiplicity 6.1 6.0 4.6
I/sigma 21.2 77.4 1.2
Rmerge 0.039 0.013 0.903
CC half 1.0 1.0 0.739
Anomalous completeness 85.0 82.6 77.8
Anomalous multiplicity 3.3 3.3 3.3
Cell 161.51 161.51 139.73 90.0 90.0 120.0

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.9795

Sweep:

SWEEP1

Files:

/programs/datagrid/456/I3_1_#####.cbf

Images:

1 - 357

Beam:

(217.32, 211.82) -> (217.32, 211.73)

Distance:

400.0 -> 399.72

Spacegroup:

P 63 2 2

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 1.74 4.72 1.74
Low resolution limit 52.74 52.76 1.77
Completeness 88.9 90.1 70.5
Multiplicity 5.3 5.9 2.4
I/sigma 14.2 49.6 1.0
Rmerge 0.042 0.018 0.616
CC half 0.999 0.999 0.49
Anomalous completeness 79.3 82.9 51.4
Anomalous multiplicity 2.9 2.9 2.9
Cell 161.206 161.206 139.485 90.0 90.0 120.0

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.