X-Ray Diffraction data from PilB from Geobacter metallireducens bound to AMP-PNP, source of 5TSH structure

Data DOI: 10.15785/SBGRID/453 | ID: 453

Publication DOI: 10.1038/ncomms15091

5TSH Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Howell Laboratory, University of Toronto

Release Date: 13 Jun 2017

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/453

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/453 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/453 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/453 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/453 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 453 ; shasum -c files.sha'

Storage requirements: 24G

Biological Sample:

PilB from Geobacter metallireducens bound to AMP-PNP

Dataset Type:

X-Ray Diffraction

Subject Composition:

Protein

Collection Facility:

08ID-1, CLS, SK, CANADA

Data Creation Date:

28 Jul 2016

Related Datasets:

None


Cite this Dataset

McCallum, M; Howell, P; Howell, L. 2017. "X-Ray Diffraction data for: PilB from Geobacter metallireducens bound to AMP-PNP. PDB Code 5TSH", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/453.

Download Citation

Dataset Description

Native dataset

Project Members

Name Additional Roles Affiliation While Working on the Project
Matthew McCallumData Collector, DepositorHospital for Sick Children
P. Lynne HowellData CollectorHospital for Sick Children
Lynne HowellPIUniversity of Toronto

Reprocessing Instructions

Processed as P1 in XDS to 2.3 A

Reprocessing Data

About Reprocessing

Version:

version unreported

Reprocessing failed.

Version:

version unreported

Reprocessing failed.

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97949

Sweep:

SWEEP1

Files:

/programs/datagrid/453/Matty18_####.img

Images:

1 - 761

Beam:

(150.97, 148.9) -> (150.97, 148.51)

Distance:

320.0 -> 320.93

Spacegroup:

P 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.22 6.03 2.22
Low resolution limit 91.52 91.6 2.26
Completeness 97.8 99.3 90.3
Multiplicity 2.1 2.1 2.0
I/sigma 5.4 9.0 1.7
Rmerge 0.111 0.074 0.554
CC half 0.975 0.987 0.474
Anomalous completeness 87.4 97.0 67.7
Anomalous multiplicity 1.1 1.1 1.1
Cell 74.34 99.97 109.81 67.01 73.39 88.91

Version:

version unreported

Project:

AUTOMATIC

Crystal:

DEFAULT

Sequence Length:

0

Wavelength:

SAD - 0.97949

Sweep:

SWEEP1

Files:

/programs/datagrid/453/Matty18_####.img

Images:

1 - 761

Beam:

(150.97, 148.9) -> (150.97, 148.6)

Distance:

320.0 -> 321.18

Spacegroup:

P 1

For AUTOMATIC/DEFAULT/SAD:

ALL LOW HIGH
High resolution limit 2.27 6.16 2.27
Low resolution limit 48.74 48.75 2.31
Completeness 98.1 98.1 91.6
Multiplicity 2.1 2.1 2.1
I/sigma 4.4 6.1 2.2
Rmerge 0.133 0.093 0.469
CC half 0.993 0.996 0.489
Anomalous completeness 87.9 95.4 75.9
Anomalous multiplicity 1.1 1.1 1.1
Cell 74.466 100.158 109.98 66.995 73.408 88.936

License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.